Nakazawa M, Miyagawa S, Nishibatake M, Ikeda K, Takao A
Department of Pediatric Cardiology, Heart Institute of Japan, Tokyo Women's Medical College, Japan.
Heart Vessels. 1988;4(3):136-40. doi: 10.1007/BF02058425.
Damage to premigratory cranial neural crest cells results in cardiovascular anomalies of so-called conotruncal anomaly. In one report, it was suggested that hemodynamic alteration would precede abnormal cardiovascular morphogenesis. We repeated this hemodynamic study in neural crest cell-excised chick embryos and found that there was no difference in heart rate and blood pressure between the treated embryos (n = 11; 164 +/- 3 beats per min (BPM) and 0.75 +/- 0.03 mmHg, respectively) and control embryos (n = 7; 160 +/- 6 BPM and 0.72 +/- 0.07 mmHg, respectively). The response of heart rate to acetylcholine was less (P less than 0.05) in the treated embryos (-13% +/- 2%) than in the control (-20% +/- 2%). Thus, the present data do not support the hypothesis that alterations in blood pressure and heart rate are causally related to abnormal cardiovascular morphogenesis. The developmental significance of subtle functional changes in neural crest-extirpated embryos in response to cholinergic challenge is unclear.
迁移前颅神经嵴细胞受损会导致所谓的圆锥动脉干异常的心血管畸形。在一份报告中，有人提出血液动力学改变会先于心血管形态异常发生。我们在切除神经嵴细胞的鸡胚中重复了这项血液动力学研究，发现处理过的胚胎（n = 11；心率分别为164±3次/分钟（BPM），血压为0.75±0.03 mmHg）与对照胚胎（n = 7；心率分别为160±6 BPM，血压为0.72±0.07 mmHg）之间的心率和血压没有差异。处理过的胚胎对乙酰胆碱的心率反应（-13%±2%）比对照胚胎（-20%±2%）小（P<0.05）。因此，目前的数据不支持血压和心率改变与心血管形态异常有因果关系的假设。神经嵴切除胚胎对胆碱能刺激的细微功能变化的发育意义尚不清楚。
