Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
J Mater Chem B. 2020 Aug 21;8(31):6810-6813. doi: 10.1039/d0tb00807a. Epub 2020 Jun 3.
Deterministic assembly of metallic nanoparticles (e.g. gold nanoparticles) into prescribed configurations has promising applications in many fields such as biosensing and drug delivery. DNA-directed bottom-up assembly has demonstrated unparalleled capability to precisely organize metallic nanoparticles into assemblies of designer configurations. However, the fabrication of assemblies comprising delicate nanoparticle arrangements, especially across large dimensions (e.g. micron size), has remained challenging. In this report, we have designed DNA origami hexagon tiles that are capable of assembling into higher-order networks of honeycomb arrays or tubes with dimensions up to several microns. The versatile addressability of the unit tile enables precise and periodic positioning of nanoparticles onto these higher-order DNA origami frame structures. Overall, we have constructed a series of 9 gold nanoparticle architectures with programmable configurations ranging from nanometer-sized clusters to micrometer-sized lattices. We believe these architectures shall hold great application potential in numerous biomedical fields.
确定的金属纳米粒子（例如金纳米粒子）组装成预定结构在生物传感和药物输送等许多领域具有广阔的应用前景。DNA 指导的自下而上的组装已经证明具有无与伦比的能力，可以将金属纳米粒子精确地组装成设计结构的组件。然而，由精致的纳米粒子排列组成的组件的制造，特别是在大尺寸（例如微米尺寸）方面，仍然具有挑战性。在本报告中，我们设计了能够组装成具有几微米尺寸的蜂窝状阵列或管的更高阶网络的 DNA 折纸六边形瓦片。单元瓦片的多功能寻址能力能够将纳米粒子精确且周期性地定位到这些高阶 DNA 折纸框架结构上。总体而言，我们构建了一系列具有可编程配置的 9 个金纳米粒子结构，从纳米级簇到微米级晶格。我们相信这些结构在许多生物医学领域具有很大的应用潜力。
