Zhou Shiming, Zhao Nan, Wang Jialei
Department of Urology, Liaocheng People's Hospital, Liaocheng, China.
Department of Reproductive Medicine, Liaocheng People's Hospital, Liaocheng, China.
Chem Biol Drug Des. 2020 Nov;96(5):1272-1279. doi: 10.1111/cbdd.13737. Epub 2020 Jul 26.
Gambogenic acid (GNA) is one of the main active components of Gamboge, and its anticancer role has been reported in some cancers. The study was to investigate the inhibitory effects of GNA on the proliferation and metastasis of bladder cancer (BC) cells and its potential regulatory mechanisms.
BC cell lines (BIU-87 cells, T24 cells, and J82 cells) were treated with different doses of GNA for different time, and then the effects of GNA on BC cell were examined in vitro using CCK-8 assay, apoptosis assays, and Transwell tests. NF-κB signaling activity was detected by the NF-κB p65 luciferase reporter assay. Western blot was used to detect the expressions of cIAP2, XIAP, Survivin, and p65.
GNA inhibited the viability of BC cells in vitro in a dose- and time-dependent manner and facilitated apoptosis of BC cells. Moreover, GNA could remarkably impede the migration and invasion abilities of BC cells. In terms of mechanism, GNA administration reduced the activity of NF-κB signaling and down-regulated the expressions of p65, survivin, XIAP, and cIAP2.
GNA blocks the growth and metastasis of BC cells via inhibiting the NF-κB signal transduction pathway.
藤黄酸（GNA）是藤黄的主要活性成分之一，其抗癌作用已在某些癌症中得到报道。本研究旨在探讨GNA对膀胱癌细胞（BC）增殖和转移的抑制作用及其潜在的调控机制。
用不同剂量的GNA处理BC细胞系（BIU-87细胞、T24细胞和J82细胞）不同时间，然后采用CCK-8法、凋亡检测法和Transwell试验在体外检测GNA对BC细胞的作用。通过NF-κB p65荧光素酶报告基因检测法检测NF-κB信号活性。采用蛋白质免疫印迹法检测cIAP2、XIAP、Survivin和p65的表达。
GNA在体外以剂量和时间依赖性方式抑制BC细胞的活力，并促进BC细胞凋亡。此外，GNA可显著阻碍BC细胞的迁移和侵袭能力。在机制方面，给予GNA可降低NF-κB信号活性，并下调p65、survivin、XIAP和cIAP2的表达。
GNA通过抑制NF-κB信号转导通路来阻断BC细胞的生长和转移。
