Harboe M, Closs O, Svindahl K, Deverill J
Infect Immun. 1977 May;16(2):662-72. doi: 10.1128/iai.16.2.662-672.1977.
Monospecific antisera against BCG antigen 60 were produced by all of four rabbits during immunization with precipitates containing antigen 60 cut out of gels after crossed immunoelectrophoresis. During electrolytic iodination of a crude antigen 60 preparation, preferential labeling of antigen 60 was demonstrated, and a specific radioimmunoassay was established to follow the development of anti-BCG-60 activity during immunization.
在用交叉免疫电泳后从凝胶中切下的含抗原60的沉淀物免疫期间,四只兔子全部产生了针对卡介苗抗原60的单特异性抗血清。在粗制抗原60制剂的电解碘化过程中,证实了抗原60的优先标记,并建立了一种特异性放射免疫测定法来跟踪免疫期间抗卡介苗-60活性的发展。