Closs O, Harboe M, Axelsen N H, Bunch-Christensen K, Magnusson M
Scand J Immunol. 1980;12(3):249-63. doi: 10.1111/j.1365-3083.1980.tb00065.x.
A reference system for the soluble antigens of Mycobacterium bovis, strain BCG is described. The system is based on crossed immunoelectrophoresis with intermediate gel. A commercially available immunoglobulin preparation made from rabbit anti-BCG hyperimmune serum was used as reference antibodies, while a concentrated BCG culture filtrate was used as reference antigen. The pattern obtained was highly reproducible, and most of the components were stable when the fiftyfold-concentrated culture filtrate was stored at -20 degrees C. About thirty different antigenic components were selected as reference antigens and numbered. The majority of the reference antigens were present in extracts prepared from BCG by ultrasonication or bacterial press extraction. Use of the system for studies of antigenic relationship between mycobacteria, identification and quantification of antigens, and characterization of antimycobacterial antibodies are illustrated by examples. The antigens of two preparations of tuberculin purified protein derivative (PPD) were identified. The antigen designated BCG60 was found to be a najor constituent of tuberculin PPD. Evidence is presented that this antigen is cell wall associated.
描述了一种用于卡介苗（牛分枝杆菌菌株）可溶性抗原的参考系统。该系统基于带有中间凝胶的交叉免疫电泳。一种由兔抗卡介苗超免疫血清制成的市售免疫球蛋白制剂用作参考抗体，而浓缩的卡介苗培养滤液用作参考抗原。获得的图谱具有高度可重复性，并且当五十倍浓缩的培养滤液储存在-20℃时，大多数成分是稳定的。大约三十种不同的抗原成分被选为参考抗原并进行编号。大多数参考抗原存在于通过超声处理或细菌压榨提取从卡介苗制备的提取物中。通过实例说明了该系统在分枝杆菌抗原关系研究、抗原鉴定和定量以及抗分枝杆菌抗体表征中的应用。鉴定了两种结核菌素纯化蛋白衍生物（PPD）制剂的抗原。发现命名为卡介苗60的抗原是结核菌素PPD的主要成分。有证据表明该抗原与细胞壁相关。
