Kotnova A P, Ilyin Yu V
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
Mol Biol (Mosk). 2020 May-Jun;54(3):426-434. doi: 10.31857/S0026898420030088.
Here we attempt to reconstruct the sequence of events that led to the formation of three regulatory piRNA clusters, namely, 20A, 38C and flamenco in the Drosophila melanogaster genome. Both the 38C and flamenco clusters include inverted sequences, which potentially form double-stranded RNA hairpins. We present evidence in favor of the well-known hypothesis of piRNA clusters as "transposon traps". According to this model, the presence of the only copy of the transposon in the genome indicates that its expression is suppressed by an RNA-interference mechanism immediately after the mobile element enters the piRNA cluster. We also discuss high the structural variability of piRNAs in Drosophila clusters and cases of horizontal transmobile elements between related species.
在此，我们试图重建导致果蝇基因组中形成三个调控性piRNA簇（即20A、38C和flamenco）的事件序列。38C和flamenco簇均包含反向序列，这些序列可能形成双链RNA发夹结构。我们提供证据支持将piRNA簇视为“转座子陷阱”这一广为人知的假说。根据该模型，基因组中转座子的唯一拷贝的存在表明，在移动元件进入piRNA簇后，其表达会立即被RNA干扰机制抑制。我们还讨论了果蝇簇中piRNA的高度结构变异性以及相关物种之间水平转移可移动元件的情况。
