Duchossois Family Institute, The University of Chicago, Chicago, IL 60637, USA; Department of Microbiology, The University of Chicago, Chicago, IL 60637, USA; Department of Medicine, Section of Infectious Diseases and Global Health, The University of Chicago, Chicago, IL 60637, USA.
Duchossois Family Institute, The University of Chicago, Chicago, IL 60637, USA.
Cell Host Microbe. 2020 Jul 8;28(1):134-146.e4. doi: 10.1016/j.chom.2020.05.005. Epub 2020 Jun 2.
Bacteria belonging to the Lachnospiraceae family are abundant, obligate anaerobic members of the microbiota in healthy humans. Lachnospiraceae impact their hosts by producing short-chain fatty acids, converting primary to secondary bile acids, and facilitating colonization resistance against intestinal pathogens. To increase our understanding of genomic and functional diversity between members of this family, we cultured 273 Lachnospiraceae isolates representing 11 genera and 27 species from human donors and performed whole-genome sequencing assembly and annotation. This analysis revealed substantial inter- and intra-species diversity in pathways that likely influence an isolate's ability to impact host health. These differences are likely to impact colonization resistance through lantibiotic expression or intestinal acidification, influence host mucosal immune cells and enterocytes via butyrate production, or contribute to synergism within a consortium by heterogenous polysaccharide metabolism. Identification of these specific functions could facilitate development of probiotic bacterial consortia that drive and/or restore in vivo microbiome functions.
属于毛螺菌科的细菌是健康人体微生物群中丰富的、专性厌氧的成员。毛螺菌科通过产生短链脂肪酸、将初级胆汁酸转化为次级胆汁酸以及促进定植抵抗肠道病原体来影响宿主。为了增加我们对该科成员之间基因组和功能多样性的理解，我们培养了来自人类供体的 273 个毛螺菌科分离株，代表 11 个属和 27 个种，并进行了全基因组测序组装和注释。这项分析揭示了在可能影响分离株影响宿主健康能力的途径中存在显著的种间和种内多样性。这些差异可能通过类细菌素表达或肠道酸化影响定植抵抗，通过丁酸产生影响宿主粘膜免疫细胞和肠细胞，或通过异质多糖代谢在联合体中产生协同作用。鉴定这些特定功能可以促进开发能够驱动和/或恢复体内微生物组功能的益生菌细菌联合体。
