Fuchs P A, Nagai T, Evans M G
Department of Physiology, University of Colorado Health Sciences Center, Denver 80262.
J Neurosci. 1988 Jul;8(7):2460-7. doi: 10.1523/JNEUROSCI.08-07-02460.1988.
Tall (inner) hair cells were isolated from specific locations in the chick cochlea. The electrical membrane properties of these cells were recorded using the tight-seal whole-cell technique. Depolarizing current steps elicited damped voltage oscillations that ranged in frequency from 100 to 250 Hz among cells from the middle third of the cochlea (basal cells). The current-voltage relation obtained under voltage clamp was dominated by calcium-activated potassium current in the voltage range over which these oscillations occurred. Tall hair cells isolated from the apical tip of the cochlea (apical cells) exhibited action potentials and lower frequency voltage oscillations (5-14 Hz) during depolarizing current steps. Outward currents in these cells were 20-fold slower than those found in the basal cells. These results suggest that electrical tuning of hair cells may play a role in determining the frequency selectivity of the chick cochlea.
从鸡的耳蜗特定位置分离出高（内侧）毛细胞。使用紧密封接全细胞技术记录这些细胞的电膜特性。去极化电流阶跃引发了阻尼电压振荡，在来自耳蜗中间三分之一处的细胞（基底细胞）中，振荡频率范围为100至250赫兹。在电压钳制下获得的电流-电压关系在这些振荡发生的电压范围内主要由钙激活钾电流主导。从耳蜗顶端分离出的高毛细胞（顶端细胞）在去极化电流阶跃期间表现出动作电位和较低频率的电压振荡（5 - 14赫兹）。这些细胞中的外向电流比基底细胞中的外向电流慢20倍。这些结果表明，毛细胞的电调谐可能在决定鸡耳蜗的频率选择性方面发挥作用。
