The University of Queensland Diamantina Institute, The University of Queensland, Brisbane, QLD 4102, Australia.
UQ Centre for Clinical Research, The University of Queensland, Brisbane, QLD 4029, Australia.
Cell Rep. 2020 Jun 2;31(9):107702. doi: 10.1016/j.celrep.2020.107702.
To better understand the influence of ultraviolet (UV) irradiation on the initial steps of skin carcinogenesis, we examine patches of labeled keratinocytes as a proxy for clones in the interfollicular epidermis (IFE) and measure their size variation upon UVB irradiation. Multicolor lineage tracing reveals that in chronically irradiated skin, patches near hair follicles (HFs) increase in size, whereas those far from follicles do not change. This is explained by proliferation of basal epidermal cells within 60 μm of HF openings. Upon interruption of UVB, patch size near HFs regresses significantly. These anatomical differences in proliferative behavior have significant consequences for the cell of origin of basal cell carcinomas (BCCs). Indeed, a UV-inducible murine BCC model shows that BCC patches are more frequent, larger, and more invasive near HFs. These findings have major implications for the prevention of field cancerization in the epidermis.
为了更好地理解紫外线（UV）辐射对皮肤癌发生初始步骤的影响，我们以标记角质形成细胞斑作为毛囊间表皮（IFE）中克隆的替代物进行检查，并测量它们在 UVB 照射下的大小变化。多色谱系追踪显示，在慢性辐射的皮肤中，靠近毛囊（HFs）的斑块会增大，而远离毛囊的斑块则不会改变。这是由于 HF 开口附近的基底表皮细胞增殖所致。中断 UVB 后，HF 附近斑块的大小显著缩小。这种增殖行为的解剖学差异对基底细胞癌（BCC）的起源细胞有重大影响。事实上，一种 UV 诱导的小鼠 BCC 模型表明，BCC 斑块在靠近 HFs 时更频繁、更大且更具侵袭性。这些发现对预防表皮的区域性癌变具有重大意义。
