The University of Queensland, UQ Diamantina Institute, Brisbane, QLD, Australia.
STAR Protoc. 2021 Feb 5;2(1):100329. doi: 10.1016/j.xpro.2021.100329. eCollection 2021 Mar 19.
The origin of basal cell carcinomas (BCC) remains elusive. This protocol combines the use of an ultraviolet (UV)-inducible BCC murine model (K14CrexPtch) and an optimized protocol for florescent whole-mount cytokeratin 17 immunostaining to address the spatiotemporal dynamics of BCC formation. The high-resolution three-dimensional confocal images are an excellent tool to visualize and quantify the distribution of skin cancers at a given time point. For complete details on the use and execution of this protocol, please refer to Roy et al. (2020).
基底细胞癌（BCC）的起源仍然难以捉摸。本方案结合了紫外线（UV）诱导的 BCC 小鼠模型（K14CrexPtch）和优化的荧光全铺角蛋白 17 免疫染色方案，以解决 BCC 形成的时空动态问题。高分辨率三维共聚焦图像是可视化和定量分析给定时间点皮肤癌分布的极好工具。有关该方案使用和执行的完整详细信息，请参考 Roy 等人。（2020 年）。
