Walicke P A
Developmental Neurobiology Laboratory, Salk Institute for Biological Studies, San Diego, California.
J Neurosci. 1988 Jul;8(7):2618-27. doi: 10.1523/JNEUROSCI.08-07-02618.1988.
Basic fibroblast growth factor (bFGF) supports the survival of neurons from many regions of the E18 fetal rat brain. Survival was significantly increased for neurons derived from the hippocampus, entorhinal cortex (EC), frontal cortex, parietal cortex (PC), occipital cortex, striatum, septum, and thalamus, but not from the subiculum (Sb). The proportion of neurons rescued by bFGF varied among brain regions, suggesting the existence of subpopulations of responsive neurons. Like hippocampal neurons, neurons from the EC and PC required about 1 pM bFGF (10-20 pg/ml) for half-maximal response; striatal neurons, in contrast, required about 3 pM bFGF. Neurite outgrowth after 24 hr exposure was significantly increased for neurons from the hippocampus, EC, and PC, while striatal neurons had only a marginal response. Although bFGF stimulated some astrocytic proliferation in the cultures, glial contamination was maintained at 2% or less. Acidic FGF (aFGF) supported smaller numbers of neurons from each region, although it significantly increased survival of neurons from hippocampus, EC, PC, striatum, and Sb. The concentration required for half-maximal survival was around 100-300 pM (2-5 ng/ml). It appears that bFGF and aFGF are potent trophic factors for many populations of CNS neurons and could potentially play a significant role in nervous system development.
碱性成纤维细胞生长因子（bFGF）可支持来自E18胎鼠脑多个区域的神经元存活。来自海马体、内嗅皮质（EC）、额叶皮质、顶叶皮质（PC）、枕叶皮质、纹状体、隔区和丘脑的神经元存活率显著提高，但来自下托（Sb）的神经元存活率未提高。bFGF挽救的神经元比例在不同脑区有所不同，这表明存在反应性神经元亚群。与海马神经元一样，来自EC和PC的神经元半最大反应所需的bFGF约为1 pM（10 - 20 pg/ml）；相比之下，纹状体神经元所需的bFGF约为3 pM。暴露24小时后，来自海马体、EC和PC的神经元的神经突生长显著增加，而纹状体神经元只有轻微反应。尽管bFGF刺激了培养物中一些星形胶质细胞的增殖，但胶质细胞污染维持在2%或更低。酸性FGF（aFGF）支持每个区域数量较少的神经元，尽管它显著提高了来自海马体、EC、PC、纹状体和Sb的神经元的存活率。半最大存活所需的浓度约为100 - 300 pM（2 - 5 ng/ml）。看来bFGF和aFGF是许多中枢神经系统神经元群体的有效营养因子，可能在神经系统发育中发挥重要作用。
