Sun Tong, Miao Jingbo, Saleem Muhammad, Zhang Haonan, Yang Yong, Zhang Qingming
Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, Shandong, 266109, China.
Department of Biological Sciences, Alabama State University, Montgomery, AL, 36101, USA.
J Hazard Mater. 2020 Nov 5;398:122941. doi: 10.1016/j.jhazmat.2020.122941. Epub 2020 May 24.
Tebuconazole is a widely used fungicide that may impair soil health. Presently, limited information is available on the bioremediation of tebuconazole-contaminated soil using biochar as a carrier for bacteria. In this study, we firstly isolated a tebuconazole-degrading strain and identified it as Alcaligenes faecalis WZ-2. Then, we used wheat straw-derived biochar as carrier to capture strain WZ-2 to assemble microorganism-immobilized composite. Finally, we investigated the effects of strain WZ-2 and biochar-immobilized WZ-2 on tebuconazole biodegradation, microbial enzyme activities and community composition in the contaminated soil. Results showed that, as compared to control, the strain WZ-2 and biochar-immobilized WZ-2 accelerated the degradation of tebuconazole, while reducing the half-life of tebuconazole from 40.8 to 18.7 and 13.3 days in soil, respectively. However, biochar alone than control slightly retarded the degradation of tebuconazole in soil. Though tebuconazole (10 mg/kg) negatively affected the soil enzyme activities (urease, dehydrogenase, and invertase) and microbiome community structure, the biochar-immobilized WZ-2 not only accelerated the degradation of tebuconazole but also restored native soil microbial enzyme activities and microbiome community composition. Our results suggest that a compatible combination of bacteria with biochar is an attractive and efficient approach for remediation of pesticide-contaminated soil and improvement of soil biological health.
戊唑醇是一种广泛使用的杀菌剂，可能会损害土壤健康。目前，关于使用生物炭作为细菌载体对戊唑醇污染土壤进行生物修复的信息有限。在本研究中，我们首先分离出一株戊唑醇降解菌株，并将其鉴定为粪产碱菌WZ-2。然后，我们使用小麦秸秆衍生的生物炭作为载体来捕获菌株WZ-2，以组装固定化微生物复合材料。最后，我们研究了菌株WZ-2和生物炭固定化WZ-2对污染土壤中戊唑醇生物降解、微生物酶活性和群落组成的影响。结果表明，与对照相比，菌株WZ-2和生物炭固定化WZ-2加速了戊唑醇的降解，同时将土壤中戊唑醇的半衰期分别从40.8天降至18.7天和13.3天。然而，单独的生物炭比对照略微延缓了土壤中戊唑醇的降解。尽管戊唑醇（10 mg/kg）对土壤酶活性（脲酶、脱氢酶和转化酶）和微生物群落结构有负面影响，但生物炭固定化WZ-2不仅加速了戊唑醇的降解，还恢复了土壤原生微生物酶活性和微生物群落组成。我们的结果表明，细菌与生物炭的兼容组合是修复农药污染土壤和改善土壤生物健康的一种有吸引力且有效的方法。
