Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2023 Jan 22;24(3):2190. doi: 10.3390/ijms24032190.
(Graeser) (Lepidoptera: Notodontidae) is a notorious pest of poplar. Coevolution with poplars rich in plant secondary metabolites prompts to expand effective detoxification mechanisms against toxic plant secondary metabolites. Although glutathione S-transferases (GSTs) play an important role in xenobiotic detoxification in , it is unclear how GSTs act in response to toxic secondary metabolites in poplar. In this study, five gene core promoters were accurately identified by a 5' loss luciferase reporter assay, and the core promoters were significantly induced by two plant secondary metabolites in vitro. Two transcription factors, cap 'n' collar C (CncC) and aryl hydrocarbon receptor nuclear translocator (ARNT), were cloned in . MtCncC and MtARNT clustered well with other insect CncCs and ARNTs, respectively. In addition, MtCncC and MtARNT could bind the promoter and strongly improve transcriptional activity, respectively. However, MtCncC and MtARNT had no regulatory function on the promoter. Our findings revealed the molecular mechanisms of the transcription factors MtCncC and MtARNT in regulating the genes of . These results provide useful information for the control of .
白杨潜叶蛾（Graeser）（鳞翅目：木蠹蛾科）是杨树的一种恶名昭彰的害虫。与富含植物次生代谢物的杨树的共同进化促使白杨潜叶蛾扩展了对有毒植物次生代谢物的有效解毒机制。尽管谷胱甘肽 S-转移酶（GSTs）在 中对异生物质解毒起着重要作用，但 GSTs 如何响应杨树中的有毒次生代谢物的作用仍不清楚。在本研究中，通过 5'缺失荧光素酶报告基因检测准确鉴定了 5 个 基因核心启动子，并且核心启动子在体外显著被两种植物次生代谢物诱导。两个转录因子，cap 'n' collar C（CncC）和芳香烃受体核转位蛋白（ARNT）在 中被克隆。MtCncC 和 MtARNT 分别与其他昆虫 CncCs 和 ARNTs 很好地聚类。此外，MtCncC 和 MtARNT 可以结合 启动子并分别强烈提高转录活性。然而，MtCncC 和 MtARNT 对 启动子没有调控作用。我们的研究结果揭示了转录因子 MtCncC 和 MtARNT 调控 基因的分子机制。这些结果为 的防治提供了有用的信息。
