Xue Cheng, Cheng Yi, Wu Jinyou, Ke Kongliang, Miao Chundi, Chen Enfu, Zhang Luqing
Department of Endocrinology, Wenling First People's Hospital, Wenling 317500, People's Republic of China.
Department of Anorectal Surgery, Ningbo Hangzhou Bay Hospital, Ningbo 315000, People's Republic of China.
Cancer Manag Res. 2020 May 11;12:3285-3291. doi: 10.2147/CMAR.S249237. eCollection 2020.
The role of circular RNA (circRNA) in papillary thyroid cancer (PTC) is largely unknown. This study aims to determine the function and mechanism of circPRMT5 in the regulation of PTC development.
PTC tissues and cell lines were used to determine circPRMT5 expression via quantitative real-time polymerase chain reaction. Small interfering RNA (siRNA) was utilized to knock down circPRMT5. Proliferation was analyzed through CCK8 and colony formation assays. Transwell assay was performed to determine cell migration and invasion. Luciferase assay and RIP assay were carried out to analyze the interaction between circPRMT5 and miR-30c.
CircPRMT5 expression was upregulated in PTC tissues and cell lines. And circPRMT5 level was positively linked with advanced stage and lymph node metastasis. CircPRMT5 knockdown inhibited proliferation, migration and invasion while inducing apoptosis. CircPRMT5 worked as a competing endogenous RNA for miR-30c. By inhibiting miR-30c, circPRMT5 promoted the expression of E2F3.
Our findings demonstrate that circPRMT5 acts as an oncogenic circRNA to promote PTC progression via regulating miR-30c/E2F3 axis.
环状RNA（circRNA）在甲状腺乳头状癌（PTC）中的作用尚不清楚。本研究旨在确定circPRMT5在PTC发生发展调控中的功能及机制。
采用PTC组织和细胞系，通过定量实时聚合酶链反应检测circPRMT5表达。利用小干扰RNA（siRNA）敲低circPRMT5。通过CCK8和集落形成实验分析细胞增殖情况。采用Transwell实验检测细胞迁移和侵袭能力。进行荧光素酶实验和RIP实验分析circPRMT5与miR-30c之间的相互作用。
circPRMT5在PTC组织和细胞系中表达上调。circPRMT5水平与疾病晚期及淋巴结转移呈正相关。敲低circPRMT5可抑制细胞增殖、迁移和侵袭，并诱导细胞凋亡。circPRMT5作为miR-30c的竞争性内源性RNA，通过抑制miR-30c促进E2F3表达。
我们的研究结果表明，circPRMT5作为一种致癌性circRNA，通过调控miR-30c/E2F3轴促进PTC进展。
