Department of General Surgery, The First People's Hospital of Yibin, Yibin, China.
BioBank, The First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, China.
J Cell Mol Med. 2020 Mar;24(6):3431-3437. doi: 10.1111/jcmm.15019. Epub 2020 Feb 5.
CircPRTM5 is associated with cell proliferation and migration in many kinds of malignancies. However, the functions and mechanisms of CircPRTM5 in CRC progression remain unclear. We explored the role and the mechanisms of CircPRTM5 in the development of CRC. Tissues of CRC patients and matched adjacent non-tumour tissues were collected to evaluate the expression of CircPRTM5. The expression of CircPRTM5 in CRC tissues was significantly higher than that in adjacent tissues. The biological functions of CircPRTM5 in CRC were determined by overexpression and down-regulation of CircPRTM5 in CRC cells in vitro and in vivo. The results indicate that knockdown of CircPRTM5 can significantly inhibit the proliferation of CRC cells. The potential mechanisms of CircPRTM5 in CRC development were identified by RT-qPCR, Western blotting analysis and luciferase reporter assay. CircPRTM5 competitively regulates the expression of E2F3 by capillary adsorption of miR-377. CircPRMT5 regulates CRC proliferation by regulating the expression of E2F3, which affects the expression of the cell cycle-associated proteins cyclinD1 and CDK2. CircPRTM5 exerts critical regulatory role in CRC progression by sponging miR-377 to induce E2F3 expression.
CircPRTM5 与多种恶性肿瘤中的细胞增殖和迁移有关。然而，CircPRTM5 在 CRC 进展中的功能和机制仍不清楚。我们探讨了 CircPRTM5 在 CRC 发展中的作用和机制。收集 CRC 患者和匹配的相邻非肿瘤组织，以评估 CircPRTM5 的表达。CRC 组织中 CircPRTM5 的表达明显高于相邻组织。通过在体外和体内过表达和下调 CRC 细胞中的 CircPRTM5 来确定 CircPRTM5 在 CRC 中的生物学功能。结果表明，CircPRTM5 的敲低可显著抑制 CRC 细胞的增殖。通过 RT-qPCR、Western blotting 分析和荧光素酶报告基因测定鉴定 CircPRTM5 在 CRC 发生发展中的潜在机制。CircPRTM5 通过毛细管吸附 miR-377 竞争调节 E2F3 的表达。CircPRTM5 通过调节 E2F3 的表达来调节 CRC 增殖，从而影响细胞周期相关蛋白 cyclinD1 和 CDK2 的表达。CircPRTM5 通过海绵吸附 miR-377 来诱导 E2F3 表达，在 CRC 进展中发挥关键调节作用。
