Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran.
Mikrochim Acta. 2020 Jun 3;187(6):366. doi: 10.1007/s00604-020-04345-0.
An ultrasonic-assisted dispersive solid-phase microextraction was developed by a multi-stimuli responsive molecularly imprinted polymer based on chain transfer agent-modified chitosan nanoparticles for enrichment and separation of trace capecitabine in a real sample. The synthesized particles were carefully characterized and it was found that a uniform pH-sensitive imprinted polymeric shell is obtained on the surface of FeO@chitosan core with enough saturation magnetization (29 emu g). Desirable adsorption capacity (91 mg g) and high imprinting factor (IF = 3.6) toward capecitabine were exhibited by the Langmuir isotherm model. Under optimized conditions, which were achieved by experimental design, the detection limit (S/N = 3) was 1.9 ng mL. The obtained relative mean recoveries of capecitabine using high-performance liquid chromatography were in the range of 93 to 102% in a human plasma sample with RSD less than 5.5%. Graphical abstract Schematic representation of ultrasonic-assisted dispersive solid-phase microextraction by multi-stimuli responsive molecularly imprinted polymer based on chain transfer agent-modified chitosan nanoparticles and high-performance liquid chromatography for enrichment and separation of capecitabine in the real sample.
基于链转移剂修饰壳聚糖纳米粒子的多刺激响应分子印迹聚合物的超声辅助分散固相微萃取用于痕量卡培他滨在实际样品中的富集和分离。合成的颗粒经过仔细表征，发现 FeO@chitosan 核表面获得了均匀的 pH 敏感印迹聚合物壳，具有足够的饱和磁化强度（29 emu g）。通过 Langmuir 等温模型，表现出对卡培他滨的理想吸附容量（91 mg g）和高印迹因子（IF = 3.6）。在实验设计达到的最佳条件下，卡培他滨的检测限（S/N = 3）为 1.9 ng mL。使用高效液相色谱法在人血浆样品中，卡培他滨的相对平均回收率在 93%至 102%之间，相对标准偏差（RSD）小于 5.5%。
