Zhang Liping, Song Xin, Dong Yuxiao, Zhao Xiyan
School of Basic Medicine and Forensic Medicine, Henan University of Science and Technology, Luoyang 471000, China.
Foods. 2024 Sep 18;13(18):2955. doi: 10.3390/foods13182955.
A novel and environmentally friendly molecularly imprinted polymer (PCA-MIP) was successfully synthesized in an aqueous solution for the selective extraction of protocatechuic acid (PCA). In this study, a deep eutectic solvent (DES, choline chloride/methacrylic acid, 1:2, mol/mol) and chitosan were employed as the eco-friendly functional monomers. These two components interacted with PCA through hydrogen bonding, integrating a multitude of recognition sites within the PCA-MIP. Thus, the resulting PCA-MIP exhibited outstanding adsorption performance, rapid adsorption rate, and better selectivity, with a maximum binding capacity of 30.56 mg/g and an equilibrium time of 30 min. The scanning electron microscope (SEM) and Brunauer-Emmett-Teller (BET) analyses revealed that the synthesized polymers possessed a uniform morphology and substantial surface areas, which were conducive to their adsorption properties. Moreover, the PCA-MIP integrated with HPLC demonstrated its efficacy as an adsorbent for the selective extraction of PCA from mango juice. The PCA-MIP presented itself as an exemplary adsorbent, offering a highly effective and eco-friendly method for the enrichment of PCA from complex matrices.
一种新型且环境友好的分子印迹聚合物（PCA-MIP）在水溶液中成功合成，用于选择性萃取原儿茶酸（PCA）。在本研究中，一种低共熔溶剂（DES，氯化胆碱/甲基丙烯酸，1:2，摩尔/摩尔）和壳聚糖被用作环境友好型功能单体。这两种成分通过氢键与PCA相互作用，在PCA-MIP内整合了大量识别位点。因此，所得的PCA-MIP表现出出色的吸附性能、快速的吸附速率和更好的选择性，最大结合容量为30.56 mg/g，平衡时间为30分钟。扫描电子显微镜（SEM）和布鲁诺尔-埃米特-泰勒（BET）分析表明，合成的聚合物具有均匀的形态和较大的表面积，这有利于它们的吸附性能。此外，与高效液相色谱（HPLC）联用的PCA-MIP证明了其作为从芒果汁中选择性萃取PCA的吸附剂的有效性。PCA-MIP是一种典范吸附剂，为从复杂基质中富集PCA提供了一种高效且环境友好的方法。
