Tang Meng-Qiu, Lin Wei-Dong, Xian Ming-Hua, Xie Yuan-Yuan, Yang Yong-Xia, Wang Shu-Mei
Guangdong Pharmaceutical University Guangzhou 510006, China Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica, State Administration of Traditional Chinese Medicine Guangzhou 510006, China Chinese Medicine Quality Engineering Technology Research Center of Guangdong Province/Higher-Education Guangzhou 510006, China.
Guangdong Pharmaceutical University Guangzhou 510006, China.
Zhongguo Zhong Yao Za Zhi. 2020 May;45(9):2186-2192. doi: 10.19540/j.cnki.cjcmm.20200219.201.
Proton nuclear magnetic resonance(~1H-NMR) is used to investigate the effect of Renshenjian Decoction on serum and urine metabolism of type 2 diabetic rats with insulin resistance induced by high-sugar and high-fat diet combined with low-dose streptozotocin(STZ). After the successful establishment of the insulin resistance model of type 2 diabetes, administration for 35 days, the serum and urine of rats were taken. Once the ~1H-NMR data have been collected and processed, PCA and OPLS-DA were used to analyze them. The results show that: compared with the blank group, the contents of methionine, taurine, α-glucose and β-glucose in the serum of the model group increased significantly(P<0.001), while the contents of 3-hydroxybutyric acid, lactic acid and unsaturated fatty acids decreased significantly(P<0.01). In the model group, the contents of trimethylamine oxide, glycine, α-glucose, β-glucose, taurine and phosphocholine in urine increased significantly(P<0.05), while the contents of creatine, lactic acid, acetic acid and citric acid decreased significantly(P<0.05). Compared with the model group, the contents of 3-hydroxybutyric acid and unsaturated fatty acids in serum of rats in the treatment group increased significantly(P<0.05), while the contents of taurine, α-glucose and β-glucose decreased significantly(P<0.01). In the treatment group, the contents of lactic acid, taurine and creatine in urine increased significantly(P<0.05), while the contents of trimethylamine oxide, glycine, α-glucose, β-glucose and phosphocholine decreased significantly(P<0.01). The results show that Renshenjian Decoction can regulate metabolic disorder and promote the metabolic phenotype to return to the normal range. It displayed therapeutic effect on type 2 diabetic rats with insulin resistance and provided a certain scientific basis for the biological basic research of Renshenjian Decoction by improving insulin resistance in diabetes mellitus.
采用质子核磁共振波谱（1H-NMR）技术，研究人参煎剂对高糖高脂饮食联合低剂量链脲佐菌素（STZ）诱导的胰岛素抵抗2型糖尿病大鼠血清及尿液代谢的影响。成功建立2型糖尿病胰岛素抵抗模型后，给药35天，采集大鼠血清和尿液。收集并处理1H-NMR数据后，采用主成分分析（PCA）和正交偏最小二乘法判别分析（OPLS-DA）进行分析。结果显示：与空白组相比，模型组血清中蛋氨酸、牛磺酸、α-葡萄糖和β-葡萄糖含量显著升高（P<0.001），而3-羟基丁酸、乳酸和不饱和脂肪酸含量显著降低（P<0.01）。模型组尿液中氧化三甲胺、甘氨酸、α-葡萄糖、β-葡萄糖、牛磺酸和磷酸胆碱含量显著升高（P<0.05），而肌酸、乳酸、乙酸和柠檬酸含量显著降低（P<0.05）。与模型组相比，治疗组大鼠血清中3-羟基丁酸和不饱和脂肪酸含量显著升高（P<0.05），而牛磺酸、α-葡萄糖和β-葡萄糖含量显著降低（P<0.01）。治疗组尿液中乳酸、牛磺酸和肌酸含量显著升高（P<0.05），而氧化三甲胺、甘氨酸、α-葡萄糖、β-葡萄糖和磷酸胆碱含量显著降低（P<0.01）。结果表明，人参煎剂可调节代谢紊乱，促使代谢表型恢复至正常范围。其对胰岛素抵抗2型糖尿病大鼠具有治疗作用，为通过改善糖尿病胰岛素抵抗进行人参煎剂的生物学基础研究提供了一定的科学依据。
