Jia Tian-Ying, Zhang Xiao-Nan, Su Tai, Yang Xiao-Yan, Wang Hai-Li, Xin Jie-Ping, Liu Meng-Nan, Wang Jing-Kun, Li Xiang-Ri
School of Chinese Pharmacy, Beijing University of Chinese Medicine Beijing 102488, China.
Yunnan Baiyao Group Limited Ltd. Kunming 650500, China.
Zhongguo Zhong Yao Za Zhi. 2020 May;45(10):2425-2430. doi: 10.19540/j.cnki.cjcmm.20200110.201.
Thin layer chromatography, high performance liquid chromatography and multivariate statistical analysis were integrated in current study to provide a basis for the quality evaluation and the standard improvement of Paridis Rhizoma(Chinese name: Chong-lou). The results demonstrated that the primary saponins in the two authorized sources of Paridis Rhizoma were polyphyllinsⅠ, Ⅱ and Ⅶ, while the rhizome of Trillium tschonoskii an adulterant of Paridis Rhizoma was rich of polyphyllin Ⅵ. Therefore, the apparent content of polyphyllin Ⅵ plays a determinant role towards the source authentication of raw materials and decoction slices of Paridis Rhizoma, whose adulterants frequently occur in the market. Moreover, the contents of polyphyllin Ⅵ in the two authorized sources could meet the requirements of Chinese Pharmacopoeia. Therefore, we suggested that polyphyllin Ⅵ should not be omitted from the quality standard of Paridis Rhizoma in the Chinese Pharmacopoeia, and on the other side, polyphyllinsⅠ, Ⅱ and Ⅶ should be the eligible quality indicators. The study aims to sound information and evidences for the quality evaluation of Paridis Rhizoma, and also to provide a theoretical basis for the standard revision of Paridis Rhizoma in the future Chinese Pharmacopoeia.
本研究将薄层色谱法、高效液相色谱法和多元统计分析相结合，为重楼质量评价和标准提升提供依据。结果表明，重楼两个法定来源中的主要皂苷为蚤休苷Ⅰ、Ⅱ和Ⅶ，而重楼的掺伪品黑籽重楼根茎富含蚤休苷Ⅵ。因此，蚤休苷Ⅵ的表观含量对重楼原材料及饮片的来源鉴定起决定性作用，重楼掺伪品在市场上屡见不鲜。此外，两个法定来源中蚤休苷Ⅵ的含量均符合《中国药典》要求。因此，我们建议《中国药典》中重楼的质量标准不应遗漏蚤休苷Ⅵ，另一方面，蚤休苷Ⅰ、Ⅱ和Ⅶ应作为合格的质量指标。本研究旨在为重楼质量评价提供充分的信息和证据，也为未来《中国药典》中重楼标准修订提供理论依据。
