环状 RNA CSPP1 在脑胶质瘤增殖和转移中的作用。
Roles of circ-CSPP1 on the proliferation and metastasis of glioma cancer.
机构信息
Department of Internal Medicine-Neurology, Taizhou First People's Hospital, Taizhou, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 May;24(10):5519-5525. doi: 10.26355/eurrev_202005_21337.
OBJECTIVE
The aim of this study was to explore the association between circ-CSPP1 and the proliferation, invasion, and migration of glioma cancer (GC).
PATIENTS AND METHODS
Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detected circ-CSPP1 expression in GC tissues and cells. Subsequently, siRNA was transfected to suppress circ-CSPP1 expression in vitro. Cell counting kit-8 (CCK-8) assay, colony formation assay, and 5-Ethynyl-2'-deoxyuridine (EdU) staining assay were performed to examine the proliferation of GC cells. Meanwhile, transwell assay was conducted to determine the invasion and migration of GC cells. Furthermore, Western blot assay was conducted to analyze the protein expressions of E-cadherin, N-cadherin, and Vimentin.
RESULTS
Circ-CSPP1 expression was significantly up-regulated both in GC tissues and cells. GC cells with low expression of circ-CSPP1 showed significantly reduced proliferation, invasion, and migration abilities. In addition, up-regulated E-cadherin protein expression, along with down-regulated N-cadherin and Vimentin protein expressions were observed in GC cells with circ-CSPP1 siRNA treatment.
CONCLUSIONS
Circ-CSPP1 promoted the proliferation, invasion, and migration of GC cells.
目的
本研究旨在探讨环状 RNA 衔接体蛋白 1（circ-CSPP1）与脑胶质瘤（GC）细胞增殖、侵袭和迁移的关系。
方法
采用实时定量聚合酶链反应（qRT-PCR）检测 GC 组织和细胞中 circ-CSPP1 的表达。随后，采用 siRNA 转染体外抑制 circ-CSPP1 的表达。采用细胞计数试剂盒-8（CCK-8）检测、集落形成实验和 5-乙炔基-2'-脱氧尿苷（EdU）染色实验检测 GC 细胞的增殖。同时，采用 Transwell 实验检测 GC 细胞的侵袭和迁移。此外，采用 Western blot 实验分析 E-钙黏蛋白、N-钙黏蛋白和波形蛋白的蛋白表达。
结果
circ-CSPP1 在 GC 组织和细胞中均显著上调。circ-CSPP1 低表达的 GC 细胞增殖、侵袭和迁移能力明显降低。此外，circ-CSPP1 siRNA 处理后 GC 细胞中 E-钙黏蛋白蛋白表达上调，N-钙黏蛋白和波形蛋白蛋白表达下调。
结论
circ-CSPP1 促进了 GC 细胞的增殖、侵袭和迁移。
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