Department of Neurosurgery, Weifang Brain Hospital, Weifang, China.
Eur Rev Med Pharmacol Sci. 2020 Dec;24(23):12251-12257. doi: 10.26355/eurrev_202012_24017.
CircRNAs are vital factors involved in the pathological processes. This study aims to elucidate the biological functions of hsa_circ_0000337 in affecting the malignant progress of glioma.
Relative levels of hsa_circ_0000337 in 45 cases of glioma and 24 cases of normal tissues were tested. The correlation between hsa_circ_0000337 and clinical features of glioma was assessed. Proliferative and metastatic abilities of U87 and U251 cells regulated by hsa_circ_0000337 were examined by 5-Ethynyl-2'-deoxyuridine (EdU) and transwell assay, respectively. Potential molecular mechanism of hsa_circ_0000337 on regulating glioma cell functions was clarified by bioinformatic analysis, which was further verified through rescue experiments.
Hsa_circ_0000337 was highly expressed in glioma cases. Its level was correlated to poor prognosis of glioma. In vitro experiments obtained the conclusion that hsa_circ_0000337 accelerated proliferative and metastatic abilities of glioma cells. Serving as a ceRNA, hsa_circ_0000337 sponged miRNA-942-5p to upregulate MAT2A, thus inducing the malignant phenotypes of glioma.
Hsa_circ_0000337/miRNA-942-5p / MAT2A axis is responsible for the deterioration of glioma. Hsa_circ_0000337 may be a potential therapeutic target for glioma.
CircRNAs 是参与病理过程的重要因素。本研究旨在阐明 hsa_circ_0000337 影响胶质瘤恶性进展的生物学功能。
检测 45 例胶质瘤和 24 例正常组织中 hsa_circ_0000337 的相对水平。评估 hsa_circ_0000337 与胶质瘤临床特征的相关性。通过 5-乙炔基-2'-脱氧尿苷（EdU）和 Transwell 分析分别检测 hsa_circ_0000337 调节 U87 和 U251 细胞增殖和转移能力。通过生物信息学分析阐明 hsa_circ_0000337 调节胶质瘤细胞功能的潜在分子机制，并通过挽救实验进一步验证。
hsa_circ_0000337 在胶质瘤病例中高表达。其水平与胶质瘤的不良预后相关。体外实验得出结论，hsa_circ_0000337 加速了胶质瘤细胞的增殖和转移能力。作为 ceRNA，hsa_circ_0000337 吸附 miRNA-942-5p 上调 MAT2A，从而诱导胶质瘤的恶性表型。
hsa_circ_0000337/miRNA-942-5p/MAT2A 轴负责胶质瘤的恶化。hsa_circ_0000337 可能是治疗胶质瘤的潜在靶点。
