环状 RNA ciRS-7 表达对肾透明细胞癌进展的影响。
Effect of ciRS-7 expression on clear cell renal cell carcinoma progression.
机构信息
Departments of Urology, Qingdao Central Hospital, Qingdao, Shandong 266042, China.
Department of Medical Imaging, Qingdao Central Hospital, Qingdao, Shandong 266042, China.
出版信息
Chin Med J (Engl). 2020 Sep 5;133(17):2084-2089. doi: 10.1097/CM9.0000000000000867.
BACKGROUND
Circular RNA ciRS-7 has been reported to be involved in the progression of various cancers. However, ciRS-7 expression and its role in clear cell renal cell carcinoma (ccRCC) progression remains unclear. This study aimed to investigate the effect of ciRS-7 expression on ccRCC and the related signaling pathway.
METHODS
ciRS-7 expression was analyzed using quantitative reverse transcription polymerase chain reaction in 87 pairs of ccRCC and matched adjacent normal tissues. The role of ciRS-7 in ccRCC cell proliferation and invasion was determined using the cell counting kit-8 and invasion assays, respectively. Potential mechanisms underlying the role of ciRS-7 in promoting ccRCC progression were explored by Western blotting. The relationship between the expression of ciRS-7 and features of ccRCC was analyzed by the Chi-square test and progression-free survival was determined using a Kaplan-Meier plot.
RESULTS
ciRS-7 was overexpressed in ccRCC tissues compared with that in matched adjacent normal tissues. In addition, ciRS-7 up-regulation was closely associated with tumor diameter (P = 0.050), clinical stage (P = 0.009), and distant metastasis (P = 0.007). ciRS-7 knockdown in 786O and 769P cells markedly inhibited their proliferative and invasive abilities. In addition, ciRS-7 inhibition reduced phosphorylated epidermal growth factor receptor (p-EGFR) and phosphorylated serine/threonine kinase (p-Akt) levels.
CONCLUSIONS
ciRS-7 up-regulation could promote ccRCC cell proliferation and invasion, which may be related with the EGFR/Akt signaling pathway. ciRS-7 might be a potential ccRCC therapeutic target.
背景
环状 RNA ciRS-7 已被报道参与多种癌症的进展。然而，ciRS-7 的表达及其在透明细胞肾细胞癌（ccRCC）进展中的作用尚不清楚。本研究旨在探讨 ciRS-7 表达对 ccRCC 及相关信号通路的影响。
方法
采用实时定量逆转录聚合酶链反应（qRT-PCR）分析 87 对 ccRCC 组织及其配对的癌旁正常组织中 ciRS-7 的表达。分别采用细胞计数试剂盒-8（CCK-8）和侵袭实验检测 ciRS-7 对 ccRCC 细胞增殖和侵袭的作用。通过 Western blot 探讨 ciRS-7 促进 ccRCC 进展的潜在机制。采用卡方检验分析 ciRS-7 表达与 ccRCC 特征的关系，采用 Kaplan-Meier 图分析无进展生存期。
结果
与配对的癌旁正常组织相比，ccRCC 组织中 ciRS-7 表达上调。此外，ciRS-7 上调与肿瘤直径（P=0.050）、临床分期（P=0.009）和远处转移（P=0.007）密切相关。在 786O 和 769P 细胞中敲低 ciRS-7 可显著抑制其增殖和侵袭能力。此外，ciRS-7 抑制降低了磷酸化表皮生长因子受体（p-EGFR）和磷酸化丝氨酸/苏氨酸激酶（p-Akt）水平。
结论
ciRS-7 上调可促进 ccRCC 细胞增殖和侵袭，这可能与 EGFR/Akt 信号通路有关。ciRS-7 可能是 ccRCC 的潜在治疗靶点。
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