Li Qianyu, Yin Min, Zhang Zhiping, Yu Yuanzhi, Liu Feng
Department of Nephrology, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China.
Evid Based Complement Alternat Med. 2022 Jul 11;2022:3729995. doi: 10.1155/2022/3729995. eCollection 2022.
Nephrotic syndrome is an enormous public healthy threaten, which causes a variety of complications and secondary disease; however, the molecular mechanism of nephrotic syndrome remains unclear.
In our study, RNA-seq were used to test the transcription level of patients with nephrotic syndrome, in order to investigate the interaction of circRNA-miRNA-mRNA in nephrotic syndrome patients.
Consistent with our hypothesis, miRNAs were confirmed to be associated with nephrotic syndrome, majority of their targeting circRNAs downregulated in nephrotic syndrome patients and at the same time, the KEGG pathway analysis found that target genes of the circRNAs bonding miRNAs was highly correlated with the occurrence of kidney diseases.
Thus, we can draw a conclusion that downregulated circRNAs cause miRNA expressing aberrant and then affect the expression level of mRNA, finally leading to the generation of nephrotic syndrome.
肾病综合征是一个巨大的公共健康威胁，它会引发多种并发症和继发性疾病；然而，肾病综合征的分子机制仍不清楚。
在我们的研究中，使用RNA测序来检测肾病综合征患者的转录水平，以研究肾病综合征患者中环状RNA-微小RNA-信使RNA的相互作用。
与我们的假设一致，微小RNA被证实与肾病综合征有关，它们的大多数靶向环状RNA在肾病综合征患者中下调，同时，京都基因与基因组百科全书通路分析发现，与微小RNA结合的环状RNA的靶基因与肾脏疾病的发生高度相关。
因此，我们可以得出结论，下调的环状RNA导致微小RNA表达异常，进而影响信使RNA的表达水平，最终导致肾病综合征的发生。
