Mohanty Varshasnata, Pinto Sneha M, Subbannayya Yashwanth, Najar Mohd Altaf, Murthy Kalpana Babu, Prasad Thottethodi Subrahmanya Keshava, Murthy Krishna R
Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore, India.
Department of vitreo retina, Vittala International Institute of Ophthalmology, Bangalore, India.
OMICS. 2020 Jun;24(6):379-389. doi: 10.1089/omi.2020.0020.
Mapping the normal eye proteome in healthy persons is essential to unravel the molecular basis of diseases impacting visual health. The vitreous occupies a large portion of the human eye between the lens and the retina and plays a significant role in vitreoretinal diseases as well as maintaining clarity in the visual field, providing nutrition to the lens, and protecting the eye from mechanical shocks. It comprises four distinct anatomical regions, namely the vitreous core, vitreous cortex, vitreous base, and anterior hyaloid. Among these, the vitreous is attached to other substructures in the eye by the vitreous base, which is its strongest point of attachment. Alterations in vitreous substructures have been reported in several vitreoretinal disorders, including vitreomacular traction, vitreoretinopathies, and age-related macular degeneration. There has been limited knowledge on proteomics variations at a resolution of vitreous substructures, including the functionally and pathophysiologically significant vitreous base. We report here new findings on the proteome map of the vitreous base in normal healthy tissue. We employed a global, unbiased proteomic profiling approach resulting in the identification of 6511 proteins. Of these, 302 proteins were involved in metabolic processes essential for energy utilization. Moreover, we identified several structural and nutrient transport proteins. Notably, the identified proteome repertoire indicates that the vitreous base might possess additional physiological functions and may not be a passive structure. This study constitutes the most extensive catalog of vitreous base proteins to our knowledge and offers novel insights as a baseline for future studies on the pathobiology of various eye diseases. These data also invite us to consider a potentially more active functional role for the vitreous base in eye physiology and visual health.
绘制健康人的正常眼蛋白质组图谱对于揭示影响视觉健康的疾病的分子基础至关重要。玻璃体占据了人眼晶状体和视网膜之间的大部分空间，在玻璃体视网膜疾病中起着重要作用，同时还能保持视野清晰、为晶状体提供营养以及保护眼睛免受机械冲击。它由四个不同的解剖区域组成，即玻璃体核心、玻璃体皮质、玻璃体基底部和前玻璃体膜。其中，玻璃体通过玻璃体基底部与眼内的其他亚结构相连，这是其最强的附着点。在几种玻璃体视网膜疾病中，包括玻璃体黄斑牵引、玻璃体视网膜病变和年龄相关性黄斑变性，都报道了玻璃体亚结构的改变。关于玻璃体亚结构分辨率下的蛋白质组学变化，包括功能和病理生理学上重要的玻璃体基底部，人们了解有限。我们在此报告正常健康组织中玻璃体基底部蛋白质组图谱的新发现。我们采用了一种全面、无偏倚的蛋白质组分析方法，鉴定出了6511种蛋白质。其中，302种蛋白质参与了能量利用所必需的代谢过程。此外，我们还鉴定出了几种结构蛋白和营养转运蛋白。值得注意的是，所鉴定的蛋白质组库表明玻璃体基底部可能具有额外的生理功能，可能不是一个被动结构。据我们所知，这项研究构成了最广泛的玻璃体基底部蛋白质目录，并为未来各种眼病病理生物学研究提供了新的见解作为基线。这些数据也促使我们考虑玻璃体基底部在眼生理学和视觉健康中可能具有更积极的功能作用。
