Laboratory of Biology of Host-Parasite Interactions, Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran.
Parasite Immunol. 2020 Sep;42(9):e12762. doi: 10.1111/pim.12762. Epub 2020 Jul 12.
Gene drive is the process of copying of an endonuclease-containing cassette that leads to increased frequency of inheritance of the desired traits in a targeted population. CRISPR/Cas9 technology is advancing genetic manipulation of insects in the field of gene drive experiments. The CRISPR/Cas9 drive could be engineered for genetic manipulation of parasites and/or vectors for disease control. A number of promising CRISPR/Cas9-based gene drive strategies that interfere with parasite development or impairs the reproductive capability of the insect vector have been proposed in the laboratory for blocking transmission of malaria and leishmaniasis. Still several technical and ethical challenges remain to be addressed, and none appear insuperable in this field.
基因驱动是一种将含有内切酶的盒式元件进行复制的过程，可导致目标种群中所需性状的遗传频率增加。CRISPR/Cas9 技术正在推动昆虫基因驱动实验领域的遗传操作。CRISPR/Cas9 驱动可用于寄生虫和/或病媒昆虫的遗传操作，以控制疾病。在实验室中已经提出了许多有前途的基于 CRISPR/Cas9 的基因驱动策略，这些策略可以干扰寄生虫的发育或削弱昆虫媒介的生殖能力，以阻断疟疾和利什曼病的传播。但是，仍然存在一些技术和伦理方面的挑战需要解决，而且在这个领域中，没有一个挑战看起来是无法克服的。
