Sánchez Victor, Brandt Annette, Jin Cheng Jun, Rajcic Dragana, Engstler Anna Janina, Jung Finn, Nier Anika, Baumann Anja, Bergheim Ina
Department of Nutritional Sciences, R.F. Molecular Nutritional Science, University of Vienna, Althanstraße 14/UZAII, A-1090 Vienna, Austria.
Institute of Nutrition, SD Model Systems of Molecular Nutrition, Friedrich-Schiller University of Jena, Dornburger Straße 25-29, 07743 Jena, Germany.
Nutrients. 2021 Mar 16;13(3):959. doi: 10.3390/nu13030959.
The addition of plant oils such as soybean oil (S) to a diet rich in saturated fatty acids is discussed as a possible route to prevent or diminish the development of metabolic disease. Here, we assessed whether a butterfat-rich diet fortified with S affects the development of early non-alcoholic steatohepatitis (NASH) and glucose intolerance. Female C57BL/6J mice were fed a standard-control diet (C); a fat-, fructose-, and cholesterol-rich diet (FFC, 25E% butterfat, 50% (wt./wt.) fructose, 0.16% (wt./wt.) cholesterol); or FFC supplemented with S (FFC + S, 21E% butterfat + 4E% S) for 13 weeks. Indicators of liver damage, inflammation, intestinal barrier function, and glucose metabolism were measured. Lipopolysaccharide (LPS)-challenged J774A.1 cells were incubated with linolenic and linoleic acids (ratio 1:7.1, equivalent to S). The development of early NASH and glucose intolerance was significantly attenuated in FFC + S-fed mice compared to FFC-fed mice associated with lower hepatic - mRNA expression, while markers of intestinal barrier function were significantly higher than in C-fed mice. Linolenic and linoleic acid significantly attenuated LPS-induced formation of reactive nitrogen species and mRNA expression in J774A.1 cells. Our results indicate that fortifying butterfat with S may attenuate the development of NASH and glucose intolerance in mice.
在富含饱和脂肪酸的饮食中添加植物油（如大豆油）被认为是预防或减轻代谢性疾病发展的一种可能途径。在此，我们评估了富含乳脂且添加了大豆油的饮食是否会影响早期非酒精性脂肪性肝炎（NASH）的发展和葡萄糖不耐受情况。将雌性C57BL/6J小鼠分为三组，分别喂食标准对照饮食（C）；富含脂肪、果糖和胆固醇的饮食（FFC，25%乳脂、50%（重量/重量）果糖、0.16%（重量/重量）胆固醇）；或添加了大豆油的FFC饮食（FFC + S，21%乳脂 + 4%大豆油），持续13周。测量了肝脏损伤、炎症、肠道屏障功能和葡萄糖代谢的指标。用脂多糖（LPS）刺激J774A.1细胞，并与亚麻酸和亚油酸（比例1:7.1，等同于大豆油）一起孵育。与喂食FFC的小鼠相比，喂食FFC + S的小鼠早期NASH和葡萄糖不耐受的发展明显减轻，同时肝脏mRNA表达较低，而肠道屏障功能标志物明显高于喂食C饮食的小鼠。亚麻酸和亚油酸显著减轻了LPS诱导的J774A.1细胞中活性氮物质的形成和mRNA表达。我们的结果表明，用大豆油强化乳脂可能会减轻小鼠NASH和葡萄糖不耐受的发展。
