Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China.
Wuxi Galaxy Biotech Co., Ltd., Wuxi, Jiangsu 214125, China.
Int J Biol Macromol. 2020 Oct 1;160:703-710. doi: 10.1016/j.ijbiomac.2020.05.263. Epub 2020 Jun 1.
Endotoxemia, a life-threatening disease affecting people worldwide, can be treated by hemoperfusion alone. New hemoperfusion materials with high biocompatibility and endotoxin-combination ability are always in demand. Herein, polymyxin B (PMB), a specific endotoxin binding molecule, was loaded onto gellan-polylysine polyion complex, and the obtained material was used in preparing wet-spun fibers. The tensile strength of the as-spun yarns (100 fibers) ranged from 1.49 N to -1.58 N and that of the dried and rewetted yarns ranged from 1.45 N to 1.56 N. The adsorption ability of the fibers with lipopolysaccharides from E. coli was 2.784 ± 0.036 EU/mg in simulated human body fluid and 2.452 ± 0.107 EU/mg in mouse plasma. The fibers showed no cytotoxicity toward U2OS cells and no hemolysis toward mouse blood. The influence of the fibers on the clotting time of mouse blood was negligible, and the blood cells were not adhesive to the fibers. Thus, the PMB-loaded gellan-polylysine complex fiber and its derivate fabrics can be used in hemoperfusion.
内毒素血症是一种影响全球人类生命安全的疾病，可以单独通过血液灌流来治疗。具有高生物相容性和内毒素结合能力的新型血液灌流材料一直是人们所需要的。在此，将一种特定的内毒素结合分子多粘菌素 B（PMB）负载到凝胶多糖-聚赖氨酸聚离子复合物上，并将得到的材料用于制备湿法纺丝纤维。所纺纤维（100 根纤维）的拉伸强度范围为 1.49 N 至-1.58 N，干燥和再润湿纤维的拉伸强度范围为 1.45 N 至 1.56 N。在模拟人体液中，纤维对大肠杆菌脂多糖的吸附能力为 2.784±0.036 EU/mg，在小鼠血浆中为 2.452±0.107 EU/mg。纤维对 U2OS 细胞无细胞毒性，对小鼠血液无溶血作用。纤维对小鼠血液凝固时间的影响可以忽略不计，并且血细胞不附着在纤维上。因此，负载 PMB 的凝胶多糖-聚赖氨酸复合纤维及其衍生的织物可用于血液灌流。
