Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Wuxi Galaxy Biotech Co., Ltd., Wuxi, Jiangsu, 214125, China.
Carbohydr Res. 2020 Oct;496:108115. doi: 10.1016/j.carres.2020.108115. Epub 2020 Aug 7.
and gellan-derived materials have never been used for suture materials due to their lack of strength and toughness. In this study, gellan and ε-polylysine formed a polyion complex in water solution, and the complex was transformed into fibers via wet-spinning. The fibers were bundled, twisted, and elongated, and the resultant twisted and elongated yarn (GPF) had a diameter of 97.53-103.76 μm and tensile strength of 4 N. The swelling ratio of GPF was 165.55%-183.23% in weight in normal saline, and the linear density was 2.84-3.31 g/km. GPF was tested using agar diffusion tests and it was found that the fibers had good antibacterial activity against Escherichia coli and Staphylococcus epidermidis. In weight loss experiments, GPF was found to be undegradable in normal saline and slightly degradable (residual weight ratio was 83.2 ± 1.2%) in simulated body fluid with trypsin within 7 days. Moreover, GPF showed no cytotoxicity toward BV-2 cells in cytotoxity tests with CCK8 and no hemolysis in hemolytic tests with fresh C57 mice blood. Finally, GPF was assessed using mouse dorsal cross-cutting model, and none of the mice that were tested with GPF showed infection or rejection reaction. Therefore, GPF is a promising suture material, and this study provides a new development direction for the application of gellan materials with improved mechanical properties.
由于其强度和韧性不足，结冷胶及其衍生材料从未被用于缝合材料。在这项研究中，结冷胶和 ε-聚赖氨酸在水溶液中形成聚离子复合物，然后通过湿法纺丝将复合物转化为纤维。纤维被束缠、扭转和拉伸，所得的扭绞和拉伸纱线（GPF）的直径为 97.53-103.76 μm，拉伸强度为 4 N。在生理盐水环境中，GPF 的溶胀比为 165.55%-183.23%，线密度为 2.84-3.31 g/km。通过琼脂扩散试验对 GPF 进行了测试，结果表明纤维对大肠杆菌和表皮葡萄球菌具有良好的抗菌活性。在失重实验中，发现 GPF 在生理盐水中不可降解，在含有胰蛋白酶的模拟体液中在 7 天内仅轻微降解（残留重量比为 83.2 ± 1.2%）。此外，在 CCK8 细胞毒性试验中，GPF 对 BV-2 细胞无细胞毒性，在新鲜 C57 小鼠血液的溶血试验中无溶血作用。最后，通过小鼠背部十字切开模型对 GPF 进行了评估，在接受 GPF 测试的所有小鼠中均未显示出感染或排斥反应。因此，GPF 是一种有前途的缝合材料，本研究为具有改善的机械性能的结冷胶材料的应用提供了新的发展方向。
