Department of Biology, Faculty of Science, Shizuoka University, 836 Ohya Suruga-ku, Shizuoka 422-8529, Japan.
Genes (Basel). 2020 Jun 2;11(6):613. doi: 10.3390/genes11060613.
INDETERMINATE DOMAIN (IDD) family proteins are plant-specific transcription factors. Some IDD (AtIDD) proteins regulate the expression of () by interacting with GRAS family transcription factors SHORT-ROOT (SHR) and SCR, which are involved in root tissue formation. Some AtIDD proteins regulate genes involved in the synthesis () or signaling () of gibberellic acid (GA) by interacting with DELLA proteins, a subfamily of the GRAS family. We analyzed the DNA binding properties and protein-protein interactions of select AtIDD proteins. We also investigated the transcriptional activity of the combination of AtIDD and GRAS proteins (AtIDD proteins combined with SHR and SCR or with REPRESSOR of (RGA)) on the promoters of , and by conducting a transient assay using culture cells. Our results showed that the promoter could be activated by the IDD and RGA complexes and that the and promoters could be activated by the IDD, SHR, and SCR complexes, indicating the possibility that these complexes regulate and consequently coordinate the expression of genes involved in GA synthesis (), GA signaling (), and root formation ().
不定域 (IDD) 家族蛋白是植物特异性转录因子。一些 IDD (AtIDD) 蛋白通过与参与根组织形成的 GRAS 家族转录因子SHORT-ROOT (SHR) 和 SCR 相互作用，调节 () 的表达。一些 AtIDD 蛋白通过与 DELLA 蛋白（GRAS 家族的一个亚家族）相互作用，调节参与赤霉素 (GA) 合成 () 或信号转导 () 的基因的表达。我们分析了选定的 AtIDD 蛋白的 DNA 结合特性和蛋白-蛋白相互作用。我们还通过使用培养细胞进行瞬时测定，研究了 AtIDD 和 GRAS 蛋白（AtIDD 蛋白与 SHR 和 SCR 或与 抑制物 (RGA) 结合）组合对 、 和 启动子的转录活性。我们的结果表明，IDD 和 RGA 复合物可以激活 启动子，而 IDD、SHR 和 SCR 复合物可以激活 和 启动子，这表明这些复合物可能调节并因此协调参与 GA 合成 ()、GA 信号转导 () 和根形成 () 的基因的表达。
