State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2023 Jun 27;24(13):10690. doi: 10.3390/ijms241310690.
is a species used in afforestation and has high economic, ecological, and therapeutic significance. experiences a variety of biotic and abiotic stresses, and thus presents a suitable model for studying how woody plants respond to such stress. Numerous families of transcription factors are involved in the research of stress resistance, with the GRAS family playing a significant role in plant development and stress response. Though s have been well explored in various plant species, much research remains to be undertaken on the family in . In this study, 21 PmGRASs were identified in the transcriptome. and phylogenetic analyses revealed that the PmGRAS family can be separated into nine subfamilies. The results of qRT-PCR and transcriptome analyses under various stress and hormone treatments reveal that PmGRASs, particularly PmGRAS9, PmGRAS10 and PmGRAS17, may be crucial for stress resistance. The majority of PmGRASs were significantly expressed in needles and may function at multiple locales and developmental stages, according to tissue-specific expression analyses. Furthermore, the DELLA subfamily members PmGRAS9 and PmGRAS17 were nuclear localization proteins, while PmGRAS9 demonstrated transcriptional activation activity in yeast. The results of this study will help explore the relevant factors regulating the development of , improve stress resistance and lay the foundation for further identification of the biological functions of PmGRASs.
是一种用于造林的物种，具有很高的经济、生态和治疗意义。 经历了各种生物和非生物胁迫，因此是研究木本植物如何应对这种胁迫的合适模型。许多转录因子家族参与了抗逆性的研究，GRAS 家族在植物发育和应激反应中起着重要作用。尽管在各种植物物种中已经对 进行了很好的研究，但在 中， 家族的研究仍有许多工作要做。在本研究中，从 转录组中鉴定出 21 个 PmGRAS。 和 系统发育分析表明，PmGRAS 家族可以分为九个亚家族。qRT-PCR 和转录组分析在各种胁迫和激素处理下的结果表明，PmGRASs，特别是 PmGRAS9、PmGRAS10 和 PmGRAS17，可能对胁迫抗性至关重要。根据组织特异性表达分析，大多数 PmGRASs 在针叶中表达显著，可能在多个部位和发育阶段发挥作用。此外，DELLA 亚家族成员 PmGRAS9 和 PmGRAS17 是核定位蛋白，而 PmGRAS9 在酵母中表现出转录激活活性。本研究的结果将有助于探索调控 发育的相关因素，提高其抗逆性，并为进一步鉴定 PmGRASs 的生物学功能奠定基础。
