Department of Chemistry, Sookmyung Women's University, Seoul 04310, Republic of Korea.
Department of Chemistry and Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul 04763, Republic of Korea.
ACS Sens. 2020 Jul 24;5(7):1943-1948. doi: 10.1021/acssensors.0c00267. Epub 2020 Jun 19.
This research introduces a method to directly detect serotonin in a single platelet through single-entity electrochemistry. Platelets isolated from human blood were analyzed by cyclic voltammetry and current-time measurements. When a single platelet collides with an ultramicroelectrode, serotonin inside the platelet is oxidized at the electrode surface, and an anodic current peak is consequently observed during measurement. The concentration of serotonin can be determined by integrating this peak current. In addition, this method can be used to determine the platelet concentration. Analysis of the collision frequency of platelets can provide information about the platelet concentration in the blood. As a result, platelet levels and serotonin concentrations in single platelets can be measured quickly and easily.
本研究介绍了一种通过单粒子电化学直接检测单个血小板中血清素的方法。通过循环伏安法和电流-时间测量对从人血中分离的血小板进行分析。当单个血小板与超微电极碰撞时，血小板内的血清素在电极表面被氧化，在测量过程中会观察到一个阳极电流峰。通过积分这个峰电流可以确定血清素的浓度。此外，这种方法还可以用来确定血小板的浓度。分析血小板的碰撞频率可以提供血液中血小板浓度的信息。因此，可以快速、轻松地测量单个血小板中的血小板水平和血清素浓度。
