Key laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology Chinese Academy of Sciences, Xining, China.
Anim Biotechnol. 2022 Feb;33(1):85-89. doi: 10.1080/10495398.2020.1773490. Epub 2020 Jun 5.
Copy number variations are primary source of genetic variations, which are associated with essential traits in many organisms. During recent years, there have been numerous research works that reveal functions of CNV. However, these studies provide only several references about copy number variations in the sheep genome. In this study, we examined the copy number variation of the gene in three Chinese sheep breeds (Chaka sheep, Hu sheep, Small-tailed Han sheep) and performed correlation analysis with growth traits, to detect the influence of CNVs. TOP2B copy numbers were divided into three distribution groups (gain, median, loss) in three Chinese sheep breeds. The distribution amount of copy number 2 of CNVs was dominant in all sheep breeds. The statistical analysis showed that CNV had a significant effect on body length in CK sheep ( 0.05), and effects on chest circumference, canon circumference ( < 0.05) in HU sheep. CNVs in STH sheep breed were relevant to chest circumference and height of hip cross ( < 0.05). These results confirmed the relationship between CNV of TOP2B gene and growth traits in three sheep breeds, and provide a reliable reference for sheep breeding.
拷贝数变异是遗传变异的主要来源，与许多生物体的重要特征有关。近年来，有大量研究揭示了 CNV 的功能。然而，这些研究仅提供了关于绵羊基因组中拷贝数变异的几个参考。在这项研究中，我们检查了三个中国绵羊品种（察哈尔羊、湖羊、小尾寒羊）中基因的拷贝数变异，并与生长性状进行了相关分析，以检测 CNV 的影响。TOP2B 基因的拷贝数在三个中国绵羊品种中分为三个分布组（增益、中位数、缺失）。在所有绵羊品种中，CNVs 的拷贝数 2 的分布量占主导地位。统计分析表明，CNV 对 CK 绵羊的体长有显著影响（0.05），对 HU 绵羊的胸围、 canon 周长有显著影响（0.05）。STH 绵羊品种的 CNVs 与胸围和髋骨交叉高度相关（0.05）。这些结果证实了 TOP2B 基因的 CNV 与三个绵羊品种的生长性状之间的关系，为绵羊育种提供了可靠的参考。
