Tianjin Key Laboratory of Structure and Performance for Functional Molecules, MOE Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry, College of Chemistry, Tianjin Normal University, 393 Binshui West Road, Tianjin, 300387, PR China.
Tianjin Key Laboratory of Structure and Performance for Functional Molecules, MOE Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry, College of Chemistry, Tianjin Normal University, 393 Binshui West Road, Tianjin, 300387, PR China.
Talanta. 2020 Sep 1;217:121019. doi: 10.1016/j.talanta.2020.121019. Epub 2020 Apr 13.
Folic acid (FA, Vitamin B9), as a water-soluble chemical compound of the vitamin B groups, plays an important role to human health. Thus, it is of great significance to develop sensitive assays for detection of FA. Herein, we prepared a water-stable metal-organic frameworks {[Ag(μ-abtz)(μ-abtz)(ClO)]⋅(ClO)} (MOF-AgClO-abtz; abtz = 1-(4-aminobenzyl)-1, 2, 4,-triazole) material, which exhibited a high fluorescent quantum yield of 78.97%, good water dispersibility and excellent chemical stability. Then MOF-AgClO-abtz was developed as a "turn-off" fluorescent sensor for detection of FA via inner filter effect (IFE). The IFE mechanism was mainly originated from the spectrum overlap between fluorescence emission of MOF-AgClO-abtz at 350 nm and UV-vis absorption band of FA ranging from 340 to 360 nm, resulting in fluorescence quenching. Furthermore, the proposed method exhibited a good linear response to FA in the concentration range of 0.1-30 μM, and the limit of detection (LOD) was calculated to be 49 nM (S/N = 3). The great reproducibility of the method was checked for three replicate detections of 20 μM FA to find relative standard deviation (RSD) of 3.4%. This method also exhibited excellent long-term stability by detecting 30 μM FA for 7 days, the RSD value was obtained to be 2.8%. Ultimately, this method was employed for sensitive detection of FA in human serum and plasma samples with quantitative spike recoveries ranging from 95.3% to 104.6%.
叶酸（FA，维生素 B9）作为维生素 B 族的一种水溶性化合物，对人体健康起着重要作用。因此，开发用于 FA 检测的灵敏分析方法具有重要意义。在这里，我们制备了一种水稳定的金属有机骨架{[Ag(μ-abtz)(μ-abtz)(ClO)]·(ClO)}（MOF-AgClO-abtz；abtz=1-(4-氨基苄基)-1,2,4,-三唑）材料，其具有 78.97%的高荧光量子产率、良好的水分散性和优异的化学稳定性。然后，MOF-AgClO-abtz 被开发为一种通过内滤效应（IFE）检测 FA 的“关闭”荧光传感器。IFE 机制主要源于 MOF-AgClO-abtz 在 350nm 处的荧光发射与 FA 的 UV-vis 吸收带（340nm 至 360nm）之间的光谱重叠，导致荧光猝灭。此外，该方法对 FA 的浓度范围在 0.1-30μM 之间表现出良好的线性响应，检测限（LOD）计算为 49nM（S/N=3）。该方法的重现性良好，通过检测 20μM FA 的三个重复检测，相对标准偏差（RSD）为 3.4%。这种方法还表现出优异的长期稳定性，通过检测 30μM FA 7 天，得到的 RSD 值为 2.8%。最终，该方法用于人血清和血浆样品中 FA 的灵敏检测，定量加标回收率范围为 95.3%至 104.6%。
