Aptamer AS1411 utilized for super-resolution imaging of nucleolin.

Nucleolin (NCL) is a multifunctional protein that mainly localizes in the nucleolus and also distributes in the nucleoplasm, cytoplasm and cell membrane. Most studies focus on its biofunctions in cell activities and diseases, however, its detailed distribution and organization pattern in situ remains obscure. Moreover, antibodies were commonly used to investigate NCL in cells. It is worth noting that antibody labeling of intracellular proteins needs detergents to permeabilize the membrane, which could disrupt the membrane structure and proteins. The emergence of aptamer AS1411 provides us a good choice to recognize the NCL without permeabilization owing to its superior cellular uptake and enhanced stability. Therefore, we applied aptamer AS1411 to super-resolution imaging to visualize the distribution of NCL at a nanometer level. Aptamer achieved a better recognition of intracellular NCL and displayed the detailed structure of NCL in different parts of cells. Significantly, cytoplasmic and membrane NCL have higher expression and larger clusters in cancer cells than that in normal cells. Our work presented a detailed organization of NCL in cells and revealed the distribution differences between cancer cells and normal cells, which promote the understanding of its functions in physiology and pathology.