Department of Biological Psychology, Technische Universität Dresden, Dresden, Germany.
Department of Biological Psychology, Technische Universität Dresden, Dresden, Germany.
Talanta. 2020 Sep 1;217:121006. doi: 10.1016/j.talanta.2020.121006. Epub 2020 Apr 9.
The endocannabinoid system has been implicated in the dynamic regulation of the stress response, fear memory formation, and inflammatory processes. Endocannabinoids (eCBs) and N-acylethanolamines (NAEs) are primarily quantified from serum or cerebrospinal fluid representing acute measures, while no validated method for the quantification of long-term integrated eCBs and NAEs concentrations exists. We here present an online solid phase extraction-liquid chromatography-mass spectrometry method (LC-MS/MS) for quantification of long-term integrated eCBs and NAEs in human hair and examine their association with burnout, depression, and anxiety symptoms. Hair samples were washed with isopropanol and endocannabinoids were extracted from 7.5 mg hair by methanol incubation. A column switching strategy for online solid phase extraction (SPE) was applied, followed by mass spectrometer detection. eCBs and NAEs levels were determined in 207 hair samples from an ongoing longitudinal study and related to individual burnout, depression and anxiety symptoms. The limits of detection were between 0.06 and 6.0 pg mg, the recoveries of this method were between 87.2% and 114.2%. Hair AEA levels showed a negative correlation with burnout and anxiety symptoms. Participants with clinically relevant burnout and anxiety symptomatology exhibited lower hair AEA levels compared to those participants with low burnout and anxiety symptomatology, while for depressive symptomatology no association was identified. The presented LC-MS/MS method provides a highly specific analytical strategy for the detection of eCBs and NAEs concentrations in human hair and is thus likely to further shed light on the temporal dynamics of eCBs and NAEs secretion. The analysis of eCBs and NAEs in hair emerges as useful strategy in biopsychological research and as a valid and easily implementable method for the retrospective assessment of cumulative long-term eCBs and NAEs secretion.
内源性大麻素系统参与了应激反应、恐惧记忆形成和炎症过程的动态调节。内源性大麻素 (eCBs) 和 N-酰基乙醇胺 (NAEs) 主要从血清或脑脊液中进行定量分析，代表急性测量，而不存在用于定量长期整合 eCBs 和 NAEs 浓度的验证方法。我们在此介绍一种用于定量人发中长期整合 eCBs 和 NAEs 的在线固相萃取-液相色谱-质谱法 (LC-MS/MS)，并研究其与倦怠、抑郁和焦虑症状的关系。毛发样本用异丙醇洗涤，甲醇孵育从 7.5mg 毛发中提取内源性大麻素。应用柱切换策略进行在线固相萃取 (SPE)，然后用质谱仪检测。在一项正在进行的纵向研究中，对 207 份毛发样本进行了 eCBs 和 NAEs 水平的测定，并与个体倦怠、抑郁和焦虑症状相关联。检测限在 0.06 到 6.0pgmg 之间，该方法的回收率在 87.2%到 114.2%之间。毛发 AEA 水平与倦怠和焦虑症状呈负相关。与低倦怠和焦虑症状的参与者相比，具有临床相关倦怠和焦虑症状的参与者的毛发 AEA 水平较低，而与抑郁症状则没有关联。本研究中建立的 LC-MS/MS 方法为检测人发中 eCBs 和 NAEs 浓度提供了一种高特异性的分析策略，因此可能进一步揭示 eCBs 和 NAEs 分泌的时间动态。eCBs 和 NAEs 在头发中的分析为生物心理研究提供了一种有用的策略，并且作为一种有效的、易于实施的方法，可以用于回溯性评估长期的 eCBs 和 NAEs 分泌。
