Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, PR China; Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, PR China.
Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, PR China; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China.
Talanta. 2020 Sep 1;217:121015. doi: 10.1016/j.talanta.2020.121015. Epub 2020 Apr 16.
Increased adoption of HPV testing is expected in light of a growing global awareness of women's health and the recent launch of cervical cancer vaccines. Testing approaches must be both easy to implement, and offer intelligible representation of amplification results. Here, we introduce a simple, rapid, and visual HPV detection method based on a loop-mediated isothermal amplification (LAMP) assay. The visual LAMP assay can be successfully applied to amplify and differentiate the high-risk samples of HPV16 and HPV18, and results can be discriminated via the naked eye, without costly specialized apparatus. This HPV testing method has been evaluated with clinical samples and exhibited excellent reliability, as verified by polymerase chain reaction-microchip electrophoresis (PCR-MCE), and has a reduced false negative rate compared with cytological methods. The LAMP-based platforms with their facile operation and visual results possess great potential for on-site cervical cancer monitoring.
鉴于人们对女性健康的认识不断提高，以及最近宫颈癌疫苗的推出，预计 HPV 检测的采用率将会增加。检测方法必须既易于实施，又能清晰地表示扩增结果。在这里，我们介绍了一种基于环介导等温扩增（LAMP）的简单、快速和可视化 HPV 检测方法。该可视化 LAMP 检测方法可成功扩增和区分 HPV16 和 HPV18 的高危样本，并且可以通过肉眼进行区分，无需昂贵的专用仪器。该 HPV 检测方法已通过临床样本进行评估，其可靠性通过聚合酶链反应-微芯片电泳（PCR-MCE）得到验证，与细胞学方法相比，其假阴性率较低。基于 LAMP 的平台操作简便，结果直观，具有很大的现场宫颈癌监测潜力。
