Rui Qingchen, Wang Junxia, Li Yanbin, Tan Xiaoyun, Bao Yiqun
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Biochem Biophys Res Commun. 2020 Jul 30;528(3):447-452. doi: 10.1016/j.bbrc.2020.05.189. Epub 2020 Jun 2.
The conserved oligomeric Golgi (COG) complex, which consists of eight subunits named COG1-COG8, is highly conserved with homologous subunits present in most eukaryotic species. In yeast and mammalian, the COG complex has been implicated in the tethering of retrograde intra-Golgi vesicles. Although homologs of COG subunits have been identified in Arabidopsis, the functions of the complex and its subunits remain to be fully elucidated. In this study, we have utilized genetic and cytologic approaches to characterize the role of the COG6 subunit. We showed that a mutation in COG6 caused male transmission defect due to aberrant pollen tube growth. At the subcellular level, Golgi bodies exhibited altered morphology in cog6 pollen and cell wall components were incorrectly deposited in pollen tubes. COG6 fused to green fluorescent protein (GFP), which complemented the aberrant growth of cog6 pollen tubes, was localized to the Golgi apparatus. We propose that COG6, as a subunit of the COG complex, modulates Golgi morphology and vesicle trafficking homeostasis during pollen tube growth.
保守寡聚高尔基体（COG）复合体由名为COG1 - COG8的八个亚基组成，在大多数真核生物物种中具有高度保守的同源亚基。在酵母和哺乳动物中，COG复合体与高尔基体逆行囊泡的拴系有关。虽然在拟南芥中已鉴定出COG亚基的同源物，但该复合体及其亚基的功能仍有待充分阐明。在本研究中，我们利用遗传学和细胞学方法来表征COG6亚基的作用。我们发现，COG6中的一个突变由于花粉管生长异常导致雄性传递缺陷。在亚细胞水平上，高尔基体在cog6花粉中呈现出形态改变，并且细胞壁成分在花粉管中沉积错误。与绿色荧光蛋白（GFP）融合的COG6定位于高尔基体，它能够弥补cog6花粉管的异常生长。我们提出，作为COG复合体的一个亚基，COG6在花粉管生长过程中调节高尔基体形态和囊泡运输稳态。
