Lee Eun Yeong, Kim Yeseul, Koo Bonhan, Noh Geun Su, Lee Hansuek, Shin Yong
Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, 05505, Republic of Korea.
Department of Physics, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of Korea.
Sens Actuators B Chem. 2020 Oct 1;320:128351. doi: 10.1016/j.snb.2020.128351. Epub 2020 May 26.
Recent advances in nucleic acid based testing using bio-optical sensor approaches have been introduced but most are based on hybridization between the optical sensor and the bio-molecule and not on an amplification mechanism. Direct nucleic acid amplification on an optical sensor has several technical limitations, such as the sensitivity of the temperature sensor, instrument complexity, and high background signal. We here describe a novel nucleic acid amplification method based on a whispering gallery mode active resonator and discuss its potential molecular diagnostic application. By implanting nanoclusters as active compounds, this active resonator operates without tapered fiber coupling and emits a strong photoluminescence signal with low background in the wavelength of low absorption in an aqueous environment that is typical of biosensors. Our method also offers an extremely low detection threshold down to a single copy within 10 min due to the strong light-matter interaction in a nano-gap structure. We envision that this active resonator provides a high refractive index contrast for tight mode confinement with simple alignment as well as the possibility of reducing the device size so that a point-of-care system with low-cost, high-sensitivity and simplicity.
基于生物光学传感器方法的核酸检测最近取得了进展，但大多数是基于光学传感器与生物分子之间的杂交，而非基于扩增机制。在光学传感器上进行直接核酸扩增存在若干技术限制，如温度传感器的灵敏度、仪器复杂性和高背景信号。我们在此描述一种基于回音壁模式有源谐振器的新型核酸扩增方法，并讨论其潜在的分子诊断应用。通过植入纳米团簇作为活性化合物，这种有源谐振器无需锥形光纤耦合即可运行，并在生物传感器典型的水性环境中的低吸收波长下发出具有低背景的强光致发光信号。由于纳米间隙结构中强烈的光-物质相互作用，我们的方法还能在10分钟内实现低至单拷贝的极低检测阈值。我们设想，这种有源谐振器为紧密模式限制提供了高折射率对比度，具有简单的对准方式，并且有可能减小设备尺寸，从而实现低成本、高灵敏度和简单性的即时检测系统。
