硒蛋白 I 对小鼠胚胎发生至关重要。
Selenoprotein I is essential for murine embryogenesis.
机构信息
Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA.
Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA.
出版信息
Arch Biochem Biophys. 2020 Aug 15;689:108444. doi: 10.1016/j.abb.2020.108444. Epub 2020 Jun 2.
Selenoprotein I (SELENOI) is an ethanolamine phosphotransferase that catalyzes the third reaction of the Kennedy pathway for the synthesis of phosphatidylethanolamine. Since the role of SELENOI in murine embryogenesis has not been investigated, SELENOI mating pairs were used to generate global KO offspring. Of 323 weanling pups, no homozygous KO genotypes were found. E6.5-E18.5 embryos (165 total) were genotyped, and only two E18.5 KO embryos were detected with no discernable anatomical defects. To screen embryos prior to uterine implantation that occurs ~ E6, blastocyst embryos (E3.5-E4.4) were flushed from uteruses of pregnant females and analyzed for morphology and genotype. KO embryos were detected in 5 of 6 pregnant females, and 7 of the 32 genotyped embryos were found to be SELENOI KO that exhibited no overt pathological features. Overall, these results demonstrate that, except for rare cases (2/490 = 0.4%), global SELENOI deletion leads to early embryonic lethality.
硒蛋白 I (SELENOI) 是一种乙醇胺磷酸转移酶，可催化磷脂酰乙醇胺合成的 Kennedy 途径的第三个反应。由于 SELENOI 在鼠胚胎发生中的作用尚未被研究，因此使用 SELENOI 交配对来产生全局 KO 后代。在 323 只断奶幼崽中，没有发现纯合 KO 基因型。对 165 只 E6.5-E18.5 胚胎进行了基因分型，仅检测到两只 E18.5 KO 胚胎，没有明显的解剖缺陷。为了在发生于 E6 的子宫植入前筛选胚胎，从怀孕雌性的子宫中冲洗出囊胚胚胎 (E3.5-E4.4) 并进行形态和基因型分析。在 6 只怀孕雌性中的 5 只中检测到 KO 胚胎，在 32 个基因分型的胚胎中，有 7 个是 SELENOI KO，它们没有明显的病理特征。总体而言，这些结果表明，除了罕见情况（2/490=0.4%）外，全局 SELENOI 缺失导致早期胚胎致死。
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