Delivery Room, Linyi Central Hospital, Shandong Province 276400, China.
Delivery Room, Linyi Central Hospital, Shandong Province 276400, China.
Life Sci. 2020 Sep 15;257:117889. doi: 10.1016/j.lfs.2020.117889. Epub 2020 Jun 2.
Gestational diabetes mellitus (GDM) has many adverse effects on offspring, such as abnormal glycolipid metabolism, obesity, insulin resistance, mental retardation, schizophrenia and so on.
We established a GDM rat model by injecting 1% streptozotocin associated with a high-fat diet one week before pregnancy, and offspring rats were sacrificed at 8 weeks of age to obtain liver tissue for study. We used hematoxylin-eosin (HE) staining to observe liver morphological changes, Tunel staining for hepatocyte apoptosis, transmission electron microscope for liver ultrastructure, and western blot for protein expression in liver tissue.
Compared with normal offspring rats, hepatocytes of GDM offspring rats showed obvious edema, liver organ index increased, and hepatocyte apoptosis and autophagosome in the liver were significantly increased; Bax, cleaved-caspase3/caspase3, LCII, Beclin 1, P-IKBα/IKBα and P-p65/p6 protein expression in the liver were significantly increased; Bcl2, p62 and PPARγ protein expression in the liver were significantly decreased. Tau prevented the GDM-related effects in the offspring: Tau decreased hepatocyte edema (or even disappears), liver organ index, hepatocyte apoptosis and the number of autophagosomes in the liver. In addition, Tau also decreased Bax, cleaved-caspase3/caspase3, LCII, Beclin 1, P-IKBα/IKBα and P-p65/p6 protein expression, and increased Bcl2, p62 and PPARγ protein expression in the liver of GDM offspring rats.
Taurine should be considered as a potential gestational nutritional supplement to prevent liver damage in GDM offspring.
妊娠期糖尿病（GDM）会对后代产生许多不良影响，如糖脂代谢异常、肥胖、胰岛素抵抗、智力低下、精神分裂症等。
我们通过在妊娠前一周注射 1%链脲佐菌素联合高脂肪饮食建立 GDM 大鼠模型，在 8 周龄时处死后代大鼠以获取肝脏组织进行研究。我们使用苏木精-伊红（HE）染色观察肝脏形态学变化，Tunel 染色观察肝细胞凋亡，透射电镜观察肝脏超微结构，Western blot 检测肝脏组织中蛋白的表达。
与正常后代大鼠相比，GDM 后代大鼠的肝细胞出现明显水肿，肝器官指数增加，肝细胞凋亡和肝脏自噬体明显增加；Bax、cleaved-caspase3/caspase3、LCII、Beclin 1、P-IKBα/IKBα 和 P-p65/p6 蛋白在肝脏中的表达明显增加；Bcl2、p62 和 PPARγ 蛋白在肝脏中的表达明显减少。牛磺酸可预防后代 GDM 相关作用：牛磺酸可减少肝细胞水肿（甚至消失）、肝器官指数、肝细胞凋亡和肝脏自噬体数量。此外，牛磺酸还降低了 Bax、cleaved-caspase3/caspase3、LCII、Beclin 1、P-IKBα/IKBα 和 P-p65/p6 蛋白在 GDM 后代大鼠肝脏中的表达，并增加了 Bcl2、p62 和 PPARγ 蛋白在肝脏中的表达。
牛磺酸可被视为一种潜在的妊娠期营养补充剂，以预防 GDM 后代的肝脏损伤。
