Department of Reproduction, Zhangjiagang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Zhang jiagang, Suzhou, Jiangsu, China.
Mol Biol Rep. 2020 Oct;47(10):7537-7546. doi: 10.1007/s11033-020-05816-6. Epub 2020 Sep 18.
To investigate the effect of puerarin on insulin resistance and inflammation in rats with gestational diabetes mellitus (GDM). Gestational diabetic model rats were established by intraperitoneal injection of streptozotocin (25 mg/kg) combined with high-fat feeding and were randomly assigned to three groups: the control group, the GDM group, and the puerarin-treated group. Puerarin was intragastrically administered to rats daily until the offspring were born. The rats in both the GDM group and control group were administered the same volume of normal saline. Serum total cholesterol, triglycerides, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in all groups of rats were measured. Haematoxylin and eosin staining was used to evaluate morphological changes in the liver, pancreas, and adipose tissues around the reproductive organs. Western blotting was carried out to measure the protein expression of IRS-1 and inflammatory factors, including TNF-α, TLR4, MyD88 and phosphorylated NF-κB, in the adipose tissues around the reproductive organs. Puerarin had preventive effects on GDM-induced pathological changes and ameliorated glucose and lipid metabolism disorders in GDM rats. Puerarin upregulated IRS-1 expression and decreased the protein expression of TNF-α, TLR4, and MyD88 as well as the levels of phosphorylated NF-κB in adipose tissues around the reproductive organs in GDM rats. This study indicated that puerarin exerts anti-inflammatory effects by downregulating the important TLR4/MyD88/NF-κB inflammatory signalling pathway. Therefore, puerarin can decrease the expression of TNF-α and ameliorate insulin resistance in GDM rats, suggesting the potential efficacy of puerarin in GDM treatment.
研究葛根素对妊娠期糖尿病（GDM）大鼠胰岛素抵抗和炎症的影响。
采用腹腔注射链脲佐菌素（25mg/kg）联合高脂饮食建立 GDM 大鼠模型，将造模成功的大鼠随机分为对照组、GDM 组和葛根素治疗组，葛根素治疗组大鼠给予葛根素灌胃，GDM 组和对照组大鼠给予等体积生理盐水，直至子代大鼠出生。检测各组大鼠血清总胆固醇、三酰甘油、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇水平，苏木精-伊红（HE）染色观察肝、胰腺和生殖器官周围脂肪组织的形态学变化，Western blot 检测生殖器官周围脂肪组织中胰岛素受体底物-1（IRS-1）和炎症因子肿瘤坏死因子-α（TNF-α）、Toll 样受体 4（TLR4）、髓样分化因子 88（MyD88）及磷酸化核因子-κB（p-NF-κB）的蛋白表达。
葛根素对 GDM 大鼠的病理性改变具有预防作用，并改善了 GDM 大鼠的糖脂代谢紊乱。葛根素可上调 IRS-1 表达，下调 GDM 大鼠生殖器官周围脂肪组织中 TNF-α、TLR4、MyD88 及 p-NF-κB 的蛋白表达水平。
葛根素通过下调 TLR4/MyD88/NF-κB 炎症信号通路发挥抗炎作用，降低 TNF-α的表达，改善 GDM 大鼠胰岛素抵抗，提示葛根素可能具有治疗 GDM 的作用。
