Institute of Food Safety and Environment Monitoring, College of Chemistry, Fuzhou University, Fuzhou 350002, PR China.
Institute of Food Safety and Environment Monitoring, College of Chemistry, Fuzhou University, Fuzhou 350002, PR China.
J Inorg Biochem. 2020 Aug;209:111100. doi: 10.1016/j.jinorgbio.2020.111100. Epub 2020 May 23.
In order to effectively avoid the side effects induced by multiple components and tedious synthesis process, a simple therapy system based on one material to simultaneously realize both photothermal therapy (PTT) and photodynamic therapy (PDT) under single laser irradiation will promote the overall phototherapeutic efficiency and make the PTT/PDT system easier to operate. Here, by using transferrin (Tf) as protein template, ultrasmall CuS@transferrin nanodots (CuS@Tf NDs) were successfully synthesized through a facile one-pot protein-based biomineralization method. The obtained CuS@Tf NDs exhibited not only excellent photothermal conversion ability (34.4%) but also high photoactivated formation of reactive oxygen species (ROS) upon 980 nm near-infrared (NIR) irradiation. By loading the drug doxorubicin (DOX) to CuS@Tf NDs, a synergistic therapy system with multiple therapeutic effects combined PTT, PDT, chemotherapy (CT) and tumor targeting properties could be perfectly implemented together by CuS@Tf-DOX NDs without any complicated post-modification process. Results from the in vitro cell experiments confirmed that these CuS@Tf-DOX NDs could produce excellent effect on cancer cells with 88.5% cell inhibition rate. In comparison with the complicated systems based on "multiple-components-in-one" strategy, this therapy system based on one single material but possess multifunctional purpose is easy to operate and more suitable for clinical applications.
为了有效避免多组分诱导的副作用和繁琐的合成过程，基于一种材料的简单治疗系统，能够在单激光照射下同时实现光热治疗（PTT）和光动力治疗（PDT），将提高整体光治疗效率，使 PTT/PDT 系统更易于操作。在这里，通过使用转铁蛋白（Tf）作为蛋白质模板，通过简便的一锅法蛋白质基生物矿化法成功合成了超小的 CuS@转铁蛋白纳米点（CuS@Tf NDs）。所得的 CuS@Tf NDs 不仅表现出优异的光热转换能力（34.4%），而且在 980nm 近红外（NIR）照射下还能高效地产生活性氧（ROS）。通过负载药物阿霉素（DOX）到 CuS@Tf NDs 上，CuS@Tf-DOX NDs 无需任何复杂的后修饰过程，即可完美地实现具有多种治疗效果的协同治疗系统，结合了 PTT、PDT、化疗（CT）和肿瘤靶向特性。体外细胞实验结果证实，这些 CuS@Tf-DOX NDs 对癌细胞具有 88.5%的细胞抑制率，可产生出色的效果。与基于“多组分合一”策略的复杂系统相比，这种基于单一材料但具有多功能的治疗系统易于操作，更适合临床应用。
