Biomass Group, College of Engineering, Nanjing Agricultural University, 40 Dianjiangtai Road, Nanjing, Jiangsu 210031, China.
Biomass Group, College of Engineering, Nanjing Agricultural University, 40 Dianjiangtai Road, Nanjing, Jiangsu 210031, China.
Bioresour Technol. 2020 Sep;312:123580. doi: 10.1016/j.biortech.2020.123580. Epub 2020 May 24.
Microbial lipids were produced by both rice straw hydrolysates and recycled pretreated glycerol. First, lipid fermentation of glucose via Cryptococcus curvatus was optimized by response surface methodology. Variables were selected by Plackett-Burman design, and optimized by central composite design, achieving 4.9 g/L total lipid and 0.16 g/g lipid yield, and increased further as glucose increased from 30 to 50 g/L. Secondly, after pretreatment, 72% lignin of rice straw was removed with glucose yield increased by 2.4 times to 74% at 20% substrate and 3 FPU/g. Subsequently, its hydrolysates produced high total lipid (8.8 g/L) and lipid yield (0.17 g/g). Finally, recycled glycerol reached the maximum total lipid of 7.2 g/L and high lipid yield of 0.16 g/g. Based on the calculation, 2.9 g total lipid would be produced from 1 g rice straw and the recycled glycerol, with a similar composition to soybean oil.
微生物油脂由水稻秸秆水解物和回收的预处理甘油生产。首先，通过响应面法优化了葡萄糖通过隐球菌属发酵生产油脂。通过 Plackett-Burman 设计选择变量，并通过中心复合设计进行优化，实现了 4.9 g/L 的总油脂和 0.16 g/g 的油脂产率，随着葡萄糖从 30 增加到 50 g/L，油脂产率进一步提高。其次，经过预处理后，水稻秸秆中的 72%木质素被去除，葡萄糖产率提高了 2.4 倍，达到 20%底物和 3 FPU/g 时的 74%。随后，其水解物产生了高总油脂（8.8 g/L）和油脂产率（0.17 g/g）。最后，回收的甘油达到了 7.2 g/L 的最大总油脂和 0.16 g/g 的高油脂产率。根据计算，从 1 g 水稻秸秆和回收的甘油中可以生产 2.9 g 总油脂，其组成与大豆油相似。
