School of Chemical Engineering, Hanoi University of Science and Technology, Hanoi, Viet Nam.
School of Chemical Engineering, Hanoi University of Science and Technology, Hanoi, Viet Nam.
Chemosphere. 2021 Apr;269:129390. doi: 10.1016/j.chemosphere.2020.129390. Epub 2020 Dec 21.
This work focuses on the lime pretreatment of rice straw, agricultural waste in northeast Vietnam, for microbial lipid (bio-lipid) production. A response surface methodology approach was performed to optimize parameters for the pretreatment process. These parameters are the concentration of Ca(OH), hydrolysis temperature, and maintained time; the effect is considered bio-lipid production yield. The lipid yield was estimated through consolidated bioprocessing of the pretreated rice straw as a substrate and using the fungus strain (Aspergillus oryzae 32) and the yeast strain (Lipomyces starkeyi 22). As a result, the optimal pretreatment conditions for maximum lipid yield were obtained at Ca(OH) concentration of 12 g/L and hydrolysis temperature of 110 °C within 60 min. The accumulated lipid in fermentation using these oleaginous microorganisms was 8.5g/100g oven-dry weight of rice straw (10.9g/100g for pretreated rice straw). The biolipid consisted of 42.6% saturated, 21.1% monounsaturated (MUFA), and 35.1% polyunsaturated (PUFA) fatty acids.
本工作聚焦于越南北部的农业废弃物稻秆的石灰预处理，以生产微生物油脂（生物油脂）。采用响应面法对预处理工艺参数进行优化。这些参数为 Ca(OH)2 浓度、水解温度和保持时间；其效果考虑为生物油脂产率。通过预处理稻秆作为底物的固态发酵，利用真菌菌株（米曲霉 32）和酵母菌株（斯达油脂酵母 22）进行整合生物加工，来估算油脂产量。结果表明，在 12g/L Ca(OH)2 浓度和 110°C 水解温度下保持 60min，可获得最大油脂产率的最佳预处理条件。使用这些产油微生物进行发酵，积累的油脂量为 100g 干重稻秆中的 8.5g（预处理稻秆为 10.9g）。生物油脂的脂肪酸组成中，饱和脂肪酸为 42.6%，单不饱和脂肪酸（MUFA）为 21.1%，多不饱和脂肪酸（PUFA）为 35.1%。
