State Key Laboratory of Fluid Power & Mechatronic Systems, College of Mechanical Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China. Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, College of Mechanical Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.
Biomed Mater. 2020 Aug 31;15(5):055036. doi: 10.1088/1748-605X/ab99d6.
An antibacterial coating with stable antibacterial properties and favorable biocompatibility is recognized as an effective method to prevent bacterial adhesion and biofilm formation on biomedical implant surfaces. In this study, a convenient and low-cost printing-spray-transfer process was proposed that enables reliably attaching antibacterial and biocompatible coatings to patient-specific silicone implant surfaces. A desktop three-dimensional printer was used to print the mold of silicone implant molds according to the characteristics of the diseased areas. Multiwalled carbon nanotubes (MWCNTs) uniformly decorated with silver nanoparticles (AgNPs/CNTs) were synthesized as the antibacterial materials for the spray process. The well-distributed AgNPs/CNT coating was anchored to the silicone surface through an in-mold transfer printing process. Stable adhesion of the coatings was assessed via tape testing and UV-vis spectra. Hardly any AgNPs/CNTs peeled off the substrate, and the adhesion was rated at 4B. Antibacterial activity, Ag release, cell viability and morphology were further assessed, revealing high antibacterial activity and great biocompatibility. The process proposed herein has potential applications for fabricating stable antibacterial coatings on silicone implant surfaces, especially for patient-specific silicone implants such as silicone tracheal stents.
具有稳定抗菌性能和良好生物相容性的抗菌涂层被认为是防止生物医学植入物表面细菌黏附和生物膜形成的有效方法。在本研究中，提出了一种方便且低成本的打印-喷涂-转移工艺，可以可靠地将抗菌和生物相容的涂层附着到患者特定的硅酮植入物表面。使用桌面式三维打印机根据病变区域的特征打印硅酮植入物模具。多壁碳纳米管（MWCNTs）均匀地被银纳米颗粒（AgNPs/CNTs）修饰，作为喷涂过程中的抗菌材料。通过模具内转移打印工艺将均匀分布的 AgNPs/CNT 涂层固定在硅酮表面。通过胶带测试和 UV-vis 光谱评估涂层的稳定附着性。几乎没有 AgNPs/CNTs 从基底剥落，其附着性评分为 4B。进一步评估了抗菌活性、Ag 释放、细胞活力和形态，结果表明具有很高的抗菌活性和良好的生物相容性。本研究提出的工艺具有在硅酮植入物表面制备稳定抗菌涂层的潜力，特别是对于患者特定的硅酮植入物，如硅酮气管支架。
