Nie Chuanxiong, Yang Ye, Cheng Chong, Ma Lang, Deng Jie, Wang Lingren, Zhao Changsheng
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China; Department of Chemistry and Biochemistry, Freie Universitat Berlin, Takustr. 3, 14195 Berlin, Germany.
Acta Biomater. 2017 Mar 15;51:479-494. doi: 10.1016/j.actbio.2017.01.027. Epub 2017 Jan 7.
The design of self-sterilizing surfaces with favorable biocompatibility is acknowledged as an effective approach to deal with the bacterial infections of biomedical devices. In this study, we report an intriguing protocol for the large-scale fabrication of self-sterilizing and biocompatible surface film coatings by using polymer shielded silver nanoparticle loaded oxidized carbon nanotube (AgNPs@oCNT) nano-dispersions. To achieve the antibacterial coatings, the bioinspired positively charged and negatively charged AgNPs@oCNTs were alternately deposited onto substrates by spray-coating assisted layer-by-layer assembly. Then the bacterial inhibitory zones, optical density value monitoring, bacterial killing efficiency and adhesion were investigated; and all the results revealed that the AgNPs@oCNTs thin film coatings exhibited robust and long-term antibacterial activity against both Gram negative and Gram positive bacteria. Moreover, due to the shielding effects of polymer layers, the coatings showed extraordinary blood compatibility and limited toxicity against human umbilical vein endothelial cells. It is believed that the proposed large-scale fabrication of bactericidal, blood and cell compatible AgNPs@oCNT based thin film coatings will have great potential to forward novel operational pathogenic inhibition strategies to avoid undesired bacterial contaminations of biomedical implants or biological devices.
Bacterial infection of medical devices has been considered to be a world-wide clinical threat towards patients' health. In this study, a bioinspired and biocompatible antibacterial coating was prepared via the spray-assisted layer-by-layer (LbL) assembly. The silver nanopartilces loaded oxidized carbon nanotube (AgNPs@oCNT), which were coated by functional polymers (chitosan and synthetic heparin mimicking polymers), were prepared via mussel inspired chemistry; and the spray-assisted assembly process allowed the fast construction on devices. Owing to the antibacterial efficiency of the loaded AgNPs, the coating showed robust bacterial killing activity and resistance towards bacterial adhesion. Moreover, since that the AgNPs were shielded by the polymers, the coating exhibited no clear toxicity at blood or cellular level. Benefiting from the universal and large-scale fabrication advancements of the spray assisted LbL coating; it is believed that the proposed strategy can be applied in designing many other kinds of self-sterilizing biomedical implants and devices.
设计具有良好生物相容性的自消毒表面被认为是应对生物医学设备细菌感染的有效方法。在本研究中，我们报告了一种有趣的方案，通过使用聚合物屏蔽的负载银纳米颗粒的氧化碳纳米管（AgNPs@oCNT）纳米分散体大规模制备自消毒和生物相容性表面薄膜涂层。为了制备抗菌涂层，通过喷雾辅助的逐层组装将具有生物启发性的带正电和带负电的AgNPs@oCNT交替沉积在基材上。然后研究了细菌抑制区、光密度值监测、细菌杀灭效率和粘附情况；所有结果表明，AgNPs@oCNT薄膜涂层对革兰氏阴性菌和革兰氏阳性菌均表现出强大且持久的抗菌活性。此外，由于聚合物层的屏蔽作用，该涂层表现出非凡的血液相容性和对人脐静脉内皮细胞的有限毒性。据信，所提出的大规模制备具有杀菌、血液和细胞相容性的基于AgNPs@oCNT的薄膜涂层将具有巨大潜力，可推动新型的操作致病抑制策略，以避免生物医学植入物或生物设备出现不必要的细菌污染。
医疗器械的细菌感染一直被认为是对患者健康的全球性临床威胁。在本研究中，通过喷雾辅助的逐层（LbL）组装制备了一种具有生物启发性和生物相容性的抗菌涂层。负载银纳米颗粒的氧化碳纳米管（AgNPs@oCNT）通过贻贝启发化学法制备，并由功能聚合物（壳聚糖和模拟合成肝素的聚合物）包覆；喷雾辅助组装过程允许在设备上快速构建。由于负载的AgNPs具有抗菌效率，该涂层表现出强大的细菌杀灭活性和对细菌粘附的抗性。此外，由于AgNPs被聚合物屏蔽，该涂层在血液或细胞水平上没有明显毒性。受益于喷雾辅助LbL涂层的通用和大规模制备进展；据信所提出的策略可应用于设计许多其他类型的自消毒生物医学植入物和设备。
