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用于检测ULBP2生物标志物的免疫传感器。

An Immunosensor for the Detection of ULBP2 Biomarker.

作者信息

Yang Wen-Chi, Liao Su-Yu, Phan Thien Luan, Hieu Nguyen Van, Chu Pei-Yi, Yi Chin-Chang, Wu Hsing-Ju, Chang Kang-Ming, Ching Congo Tak-Shing

机构信息

Department of Pathology, Show Chwan Memorial Hospital, Changhua City 500, Taiwan.

Department of Electrical Engineering, National Chi Nan University, Nantou County 545, Taiwan.

出版信息

Micromachines (Basel). 2020 Jun 3;11(6):568. doi: 10.3390/mi11060568.

DOI:10.3390/mi11060568
PMID:32503144
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7344431/
Abstract

Pancreatic cancer (PC) is a global health problem that features a very high mortality rate. The UL16 binding protein 2 (ULBP2) is a new biomarker for PC detection. This study develops a simple, reliable, and inexpensive immunosensor for the detection of the ULBP2 antigen while also investigating the effects of an array configuration of connected sensors and zinc oxide (ZnO) nanoparticles on the immunosensor's sensitivity. The ULBP2 antibody was immobilized onto the screen-printed carbon electrode (SPCE) surfaces of three different sensors: a simple SPCE (ULBP2-SPCE); an SPCE array, which is a series of identical SPCE connected to each other at different arrangements of rows and columns (ULBP2-SPCE-1x2 and ULBP2-SPCE-1x3); and an SPCE combined with ZnO nanoparticles (ULBP2-ZnO/SPCE). Impedance spectrum measurements for the immunosensors to ULBP2 antigen were conducted and compared. According to the result, the array configurations (ULBP2-SPCE-1x2 and ULBP2-SPCE-1x3) show an improvement of sensitivity compared to the ULBP2-SPCE alone, but the improvement is not as significant as that of the ULBP2-ZnO/SPCE configuration (ULBP2-ZnO/SPCE > ULBP2-SPCE: 18 times larger). The ULBP2-ZnO/SPCE immunosensor has a low limit of detection (1 pg/mL) and a high sensitivity (332.2 Ω/Log(pg/mL)), excellent linearity (R = 0.98), good repeatability (coefficients of variation = 5.03%), and is stable in long-term storage (retaining 95% activity after 28 days storage). In an array configuration, the immunosensor has an increased signal-to-noise ratio (ULBP2-SPCE-1x3 > ULBP2-SPCE: 1.5-fold) and sensitivity (ULBP2-SPCE-1x3 > ULBP2-SPCE: 2.6-fold). In conclusion, either the modification with ZnO nanoparticles onto the sensor or the use of an array configuration of sensors can enhance the immunosensor's sensitivity. In this study, the best immunosensor for detecting ULBP2 antigens is the ULBP2-ZnO/SPCE immunosensor.

摘要

胰腺癌（PC）是一个全球性的健康问题，其死亡率极高。UL16结合蛋白2（ULBP2）是一种用于胰腺癌检测的新型生物标志物。本研究开发了一种简单、可靠且廉价的免疫传感器，用于检测ULBP2抗原，同时还研究了连接传感器的阵列配置和氧化锌（ZnO）纳米颗粒对免疫传感器灵敏度的影响。将ULBP2抗体固定在三种不同传感器的丝网印刷碳电极（SPCE）表面：一个简单的SPCE（ULBP2-SPCE）；一个SPCE阵列，它是一系列以不同行和列排列方式相互连接的相同SPCE（ULBP2-SPCE-1x2和ULBP2-SPCE-1x3）；以及一个与ZnO纳米颗粒结合的SPCE（ULBP2-ZnO/SPCE）。对免疫传感器针对ULBP2抗原进行了阻抗谱测量并进行比较。结果显示，与单独的ULBP2-SPCE相比，阵列配置（ULBP2-SPCE-1x2和ULBP2-SPCE-1x3）的灵敏度有所提高，但提高幅度不如ULBP2-ZnO/SPCE配置显著（ULBP2-ZnO/SPCE > ULBP2-SPCE：大18倍）。ULBP2-ZnO/SPCE免疫传感器具有低检测限（1 pg/mL）和高灵敏度（332.2 Ω/Log(pg/mL)），线性良好（R = 0.98），重复性好（变异系数 = 5.03%），并且在长期储存中稳定（储存28天后保留95%的活性）。在阵列配置中，免疫传感器的信噪比增加（ULBP2-SPCE-1x3 > ULBP2-SPCE：1.5倍）且灵敏度提高（ULBP2-SPCE-1x3 > ULBP2-SPCE：2.6倍）。总之，在传感器上用ZnO纳米颗粒进行修饰或使用传感器的阵列配置均可提高免疫传感器的灵敏度。在本研究中，检测ULBP2抗原的最佳免疫传感器是ULBP2-ZnO/SPCE免疫传感器。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db1/7344431/b2738b11a65b/micromachines-11-00568-g013.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db1/7344431/6c7655688519/micromachines-11-00568-g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2db1/7344431/b2738b11a65b/micromachines-11-00568-g013.jpg

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