Department of Chemistry, Capital Normal University, Beijing 100048, China.
Department of Chemistry, Capital Normal University, Beijing 100048, China.
Biosens Bioelectron. 2016 Jan 15;75:148-54. doi: 10.1016/j.bios.2015.08.041. Epub 2015 Aug 20.
In this work, a one-pot method was designed to synthesize copper ions, cadmium ions, lead ions and zinc ions doped chitosan-poly(acrylic acid) nanospheres. Those nanospheres can not only produce independent electrochemical signals, but also react with glutaraldehyde (GA) to immobilize different labeled antibodies. Using the modified nanospheres as immunoprobes, a sandwich-type immunosensor was fabricated to simultaneous detection of four tumor markers (CEA, CA199, CA125 and CA242) of pancreatic cancer. This designed immunosensor exhibited good linear relationships in range from 0.1 to 100ng mL(-1) for CEA, 1 to 150UmL(-1) for CA199, CA125 and CA242, corresponding detection limits 0.02ng mL(-1), 0.4UmL(-1), 0.3UmL(-1) and 0.4UmL(-1), respectively. Meanwhile, the immunosensor was applied in analysis of clinical serum samples, whose results were well agreed with the enzyme-linked immunosorbent assay (ELISA), indicating that the proposed immunosensor gave a hope for the identification and validation of specific early cancer.
在这项工作中，设计了一种一锅法来合成铜离子、镉离子、铅离子和锌离子掺杂的壳聚糖-聚丙烯酸纳米球。这些纳米球不仅可以产生独立的电化学信号，还可以与戊二醛（GA）反应来固定不同的标记抗体。使用修饰后的纳米球作为免疫探针，构建了一种三明治型免疫传感器，用于同时检测胰腺癌的四种肿瘤标志物（CEA、CA199、CA125 和 CA242）。该设计的免疫传感器在 CEA 的检测范围为 0.1 至 100ng/mL 时，CA199、CA125 和 CA242 的检测范围为 1 至 150U/mL 时，均呈现出良好的线性关系，对应的检测限分别为 0.02ng/mL、0.4U/mL、0.3U/mL 和 0.4U/mL。同时，该免疫传感器被应用于临床血清样品的分析，其结果与酶联免疫吸附测定（ELISA）吻合良好，表明所提出的免疫传感器为特定早期癌症的鉴定和验证提供了希望。
