Laboratory of Veterinary Biochemistry and Molecular Biology, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk 28644, Korea.
Int J Mol Sci. 2020 Jun 3;21(11):4009. doi: 10.3390/ijms21114009.
Triclosan (TCS) is one of the most common endocrine-disrupting chemicals (EDCs) present in household and personal wash products. Recently, concerns have been raised about the association between abnormal behavior in children and exposure to EDC during gestation. We hypothesized that exposure to TCS during gestation could affect brain development. Cortical neurons of mice were exposed in vitro to TCS. In addition, we examined in vivo whether maternal TCS administration can affect neurobehavioral development in the offspring generation. We determined that TCS can impair dendrite and axon growth by reducing average length and numbers of axons and dendrites. Additionally, TCS inhibited the proliferation of and promoted apoptosis in neuronal progenitor cells. Detailed behavioral analyses showed impaired acquisition of spatial learning and reference memory in offspring derived from dams exposed to TCS. The TCS-treated groups also showed cognition dysfunction and impairments in sociability and social novelty preference. Furthermore, TCS-treated groups exhibited increased anxiety-like behavior, but there was no significant change in depression-like behaviors. In addition, TCS-treated groups exhibited deficits in nesting behavior. Taken together, our results indicate that perinatal exposure to TCS induces neurodevelopment disorder, resulting in abnormal social behaviors, cognitive impairment, and deficits in spatial learning and memory in offspring.
三氯生（TCS）是家用和个人洗护产品中最常见的内分泌干扰化学物质（EDCs）之一。最近，人们对儿童行为异常与妊娠期接触 EDC 之间的关联表示担忧。我们假设妊娠期接触 TCS 可能会影响大脑发育。我们将体外培养的皮质神经元暴露于 TCS 中。此外，我们还研究了母体 TCS 给药是否会影响后代的神经行为发育。结果表明，TCS 通过减少轴突和树突的平均长度和数量来损害树突和轴突的生长。此外，TCS 抑制神经元祖细胞的增殖并促进其凋亡。详细的行为分析表明，来自接触 TCS 的母体的后代在空间学习和参考记忆的获得方面存在损伤。TCS 处理组还表现出认知功能障碍以及社交和社交新颖性偏好受损。此外，TCS 处理组表现出类似焦虑的行为增加，但抑郁样行为没有明显变化。此外，TCS 处理组的筑巢行为也存在缺陷。综上所述，我们的研究结果表明，围产期接触 TCS 会导致神经发育障碍，从而导致后代出现异常的社交行为、认知障碍以及空间学习和记忆缺陷。
