Odor Alin Alexandru, Bechir Edwin Sever, Forna Doriana Agop
Department of Periodontology, Faculty of Dental Medicine, University of Titu Maiorescu, Bucharest, Romania.
Department of Oral Rehabilitation and Oclusology, Faculty of Dental Medicine, University of Medicine, Pharmacy, Science and Technology of Târgu-Mureş, Târgu-Mureş, Romania.
Photobiomodul Photomed Laser Surg. 2020 Oct;38(10):614-624. doi: 10.1089/photob.2019.4718. Epub 2020 Jun 5.
The aim of this study was to compare the antimicrobial effects of hydroxyl radical generation by photoactivation of hydrogen peroxide (HO) with diode laser (λ = 940 nm) in combination with conventional nonsurgical periodontal therapy. Thirty-eight patients and 114 teeth were included in this study. The test teeth were randomly assigned to one of the three treatment groups: Group 1 (control group): scaling and root planning (SRP); and the following experimental groups: Group 2: SRP +940 nm diode laser; Group 3: SRP+photoactivation of HO with 940 nm diode laser. Clinical examinations, such as periodontal probing depth (PPD), clinical attachment level (CAL), and bleeding on probing (BoP) were performed before and after the treatment. The microbiological evaluation included nine periodontal bacterial species investigated by means of real-time polymerase chain reaction assay before and after the treatment. The clinical and bacterial differences were assessed between the investigated groups. The total bacteria load was reduced for all three studied groups and all periodontal indexes (PPD, CAL, and BoP) were improved after each treatment. Group 3 showed significant bacterial reduction of the major periodontal bacteria such as , , , , , , ( < 0.001) in contrast to the other two groups ( > 0.001). Differences between tested groups showed significant results with regard to Group 3. The synergistic effect of SRP and photoactivation of HO with 940 nm diode laser offers an efficient and reliable antimicrobial effect in the nonsurgical periodontal treatment approach.
本研究的目的是比较过氧化氢（HO）经二极管激光（λ = 940 nm）光活化产生的羟基自由基与传统非手术牙周治疗联合使用时的抗菌效果。本研究纳入了38例患者和114颗牙齿。受试牙齿被随机分配到三个治疗组之一：第1组（对照组）：龈下刮治和根面平整（SRP）；以及以下实验组：第2组：SRP + 940 nm二极管激光；第3组：SRP + 940 nm二极管激光光活化HO。在治疗前后进行临床检查，如牙周探诊深度（PPD）、临床附着水平（CAL）和探诊出血（BoP）。微生物学评估包括在治疗前后通过实时聚合酶链反应测定法对9种牙周细菌进行研究。评估各研究组之间的临床和细菌差异。所有三个研究组的总细菌载量均降低，且每次治疗后所有牙周指标（PPD、CAL和BoP）均得到改善。与其他两组（P > 0.001）相比，第3组显示主要牙周细菌如 、 、 、 、 、 、 显著减少（P < 0.001）。测试组之间的差异在第3组方面显示出显著结果。SRP与940 nm二极管激光光活化HO的协同作用在非手术牙周治疗方法中提供了高效可靠的抗菌效果。
